KDM1 Polyclonal Antibody

Mouse Placenta lysates probed with KDM1 Polyclonal Antibody, Unconjugated (bs-10166R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.

$Lane 1: Mouse Testis lysates; Lane 2: Mouse Uterus lysates; Lane 3: Mouse NIH\/3T3 cell lysates; Lane 4: Rat Testis lysates; Lane 5: Human Hela cell lysates; Lane 6: Human 293T cell lysates; Lane 7: Human Jurkat cell lysates probed with KDM1 Polyclonal Antibody, Unconjugated (bs-10166R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Mouse Testis lysates; Lane 2: Mouse Uterus lysates; Lane 3: Mouse NIH\/3T3 cell lysates; Lane 4: Rat Testis lysates; Lane 5: Human Hela cell lysates; Lane 6: Human 293T cell lysates; Lane 7: Human Jurkat cell lysates probed with KDM1 Polyclonal Antibody, Unconjugated (bs-10166R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

$Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (KDM1\/LSD1) polyclonal Antibody, Unconjugated (bs-10166R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.$

Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (KDM1\/LSD1) polyclonal Antibody, Unconjugated (bs-10166R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Applications

WB
ELISA

Reactivity

Human
Mouse
Rat

Predicted Reactivity

Dog
Cow
Pig
Chicken
Rabbit

Overview
Catalog #	bs-10166R
Product Name	KDM1 Polyclonal Antibody
Applications	WB, ELISA
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Dog, Cow, Pig, Chicken, Rabbit
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human KDM1
Immunogen Range	301-400/852
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Subcellular location	Nucleus
Synonyms	Amine oxidase flavin containing domain 2; Amine oxidase flavin containing domain 2; Amine oxidase flavin containing domain protein 2; AOF 2; AOF2; BHC110; BRAF35 HDAC complex protein BHC110; BRAF35-HDAC complex protein BHC110; FAD binding protein BRAF35 HDAC complex, 110 kDa subunit; Flavin containing amine oxidase domain containing protein 2; Flavin-containing amine oxidase domain-containing protein 2; KDM 1; Kdm1a; KDM1A_HUMAN; KIAA0601; LSD 1; LSD1; Lysine K specic demethylase 1; Lysine K specic demethylase 1A; Lysine specic histone demethylase 1; Lysine specic histone demethylase 1A; Lysine-specic histone demethylase 1; Lysine-specic histone demethylase 1A.
Background	Gene activation and repression is specifically regulated by histone methylation status at distinct lysine residues. Lysine specific demethylase 1 (KDM1/LSD1) is a long-sought histone demethylase that specifically demethylates mono and di methyl histone H3 at K4 and K9. Thus KDM1 is a specific tag for epigenetic transcriptional activation, thereby acting as a corepressor.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000