Recombinant Protein G, Tag free
Applications
Reactivity
| Overview | |
| Catalog # | bs-0361P |
| Product Name | Recombinant Protein G, Tag free |
| Applications | ELISA |
| Reactivity | Others |
| Specifications | |
| Conjugation | Unconjugated |
| Source | Recombinant Protein G, Tag free protein is expressed in E.coli with Tag free. It contains the amino acid sequence of N/A. |
| Storage Buffer | 0.01M TBS(pH7.4) |
| Storage Condition | The product should be stored at -70°C or -20°C. |
| Target | |
| Swiss Prot | P06654 |
| Background | Protein G is an immunoglobulin-binding protein expressed in group C and G Streptococcal bacteria much like Protein A but with differing specificities. It is a 65-kDa (G148 protein G) and a 58 kDa (C40 protein G)[1] cell surface protein that has found application in purifying antibodies through its binding to the Fc region. The native molecule also binds albumin, however, because serum albumin is a major contaminant of antibody sources, the albumin binding site has been removed from recombinant forms of Protein G. Recombinant protein G has a very high affinity for the Fc regions of IgG molecules. Immoblisation of protein G on beads creates an affinity resin which can be used to purify IgG fractions from crude serum, ascites fluid or cell cuture media. |
Powered by Bioz