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DDB1 Monoclonal Antibody + Cell Lysate Kit

Applications

  • WB
  • FCM
  • IC

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bsm-90031R
Product Name DDB1 Monoclonal Antibody + Cell Lysate Kit
Applications WB, FCM, IC
Specificity Knockdown Validated
Reactivity Human, Mouse, Rat
Specifications
Conjugation Unconjugated
Host Rabbit
Source A synthesized peptide derived from human DDB1
Immunogen Range 1100-1140/1140
Clonality Monoclonal
Clone # 23GB2265
Isotype IgG
Concentration Lot dependent
Purification Affinity Purified
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Condition -20 °C
Target
Gene ID 1642
Swiss Prot Q16531
Synonyms DDB1; Damage Specific DNA Binding Protein 1; Xeroderma Pigmentosum Group E-Complementing Protein ; UV-Damaged DNA-Binding Protein 1; UV-Damaged DNA-Binding Factor; DNA Damage-Binding Protein 1; DNA Damage-Binding Protein A; HBV X-Associated Protein 1; XPE-Binding Factor; DDB P127 Subunit; UV-DDB 1; XPE-BF; XAP-1; XAP1; Damage-Specific DNA Binding Protein 1 (127kD); Damage-Specific DNA Binding Protein 1, 127kDa; Damage-Specific DNA-Binding Protein 1; UV-DDB1; WHIKERS; DDBA; XPCE; DDBa; XPCe; XPE
Background Protein, which is both involved in DNA repair and protein ubiquitination, as part of the UV-DDB complex and DCX (DDB1-CUL4-X-box) complexes, respectively. Core component of the UV-DDB complex (UV-damaged DNA-binding protein complex), a complex that recognizes UV-induced DNA damage and recruit proteins of the nucleotide excision repair pathway (the NER pathway) to initiate DNA repair. The UV-DDB complex preferentially binds to cyclobutane pyrimidine dimers (CPD), 6-4 photoproducts (6-4 PP), apurinic sites and short mismatches. Also functions as a component of numerous distinct DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. DCX(DDB2) (also known as DDB1-CUL4-ROC1, CUL4-DDB-ROC1 and CUL4-DDB-RBX1) may ubiquitinate histone H2A, histone H3 and histone H4 at sites of UV-induced DNA damage.

Cell Line: HeLa

Knockdown technology: shRNA Knockdown
Application Dilution
WB : 1:1000-1:50000
FCM FCM: 1:2000
IC IC: 1:1000