Overview |
bsm-70605M |
Phosphoserine/threonine Antibody |
WB, IP, ICC |
The antibody detects many serine or threonine phosphorylated proteins by western blot, immunocytochemistry, and ELISA. Clone M380B was generated.from a phosphoserine synthetic peptide (coupled to carrier protein). |
Human, Mouse, Rat |
Specifications |
Unconjugated |
Mouse |
PM3801 Phosphoserine/threonine is a mix of two clones: Clone M380A was generated from a phosphothreonine synthetic peptide (coupled to carrier protein) |
pSer/Thr |
Monoclonal |
M380A/M380B |
IgG1 |
Lot dependent |
Purified by Protein A. |
PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol |
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. |
Target |
N/A |
Phosphoser/thr mAb |
Phosphorylation of specific serine or threonine residues is an important post-translational modification for regulating the activity of most proteins. Stimulation of a variety of cell signaling pathways activates the receptor and non-receptor ser/thr kinases that mediate these protein modifications. Antibodies that can detect phosphoserine or phosphothreonine residues are excellent tools for characterizing changes in the post-translational state of a broad range of phosphorylated proteins. Immunoprecipitation of proteins of interest followed by detection of phosphoserine or phosphothreonine using anti-phosphoserine antibody is commonly used to correlate changes in phosphorylation state with alterations in protein activity. |
Application Dilution |
WB |
1:300-5000 |
IP |
1-2ug |
ICC |
1:100-500 |