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Cdc42 Antibody

Applications

  • WB
  • ICC

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bsm-70417M
Product Name Cdc42 Antibody
Applications WB, ICC
Specificity The antibody detects a 21 kDa* protein in human Jurkat cells, rat A7r5 cells, and mouse brain. The antibody also detects recombinant full length rat Cdc42 fusion protein, but does not recognize a full length human RhoA GST fusion protein.
Reactivity Human, Mouse, Rat
Specifications
Conjugation Unconjugated
Host Mouse
Source Clone (M430) was generated from a rat recombinant Cdc42 protein. This sequence is highly conserved in human and mouse Cdc42.
Clonality Monoclonal
Clone # M430
Isotype IgM
Purification Antigen Affinity purification
Storage Buffer PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
Storage Condition Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Target
Swiss Prot Q8CFN2
Synonyms CDC-42, G25K
Background Cdc42 proteins are Rho GTPase family members, which act as molecular switches in regulating a variety of biological response pathways including cell motility, cell-cycle progression, gene transcription, and cell transformation. Cdc42 GTPases regulate molecular events by cycling from the inactive GDP-bound state to active GTP-bound forms. The ratio of GDP versus GTP, which is bound to the GTPase, is determined by the opposing effects of guanine nucleotide exchange factors and GTPase activating proteins. Active (GTP-bound) Cdc42 binds to the p21-binding domain of p21-activated protein kinase 1 (PAK1) to regulate downstream signaling cascades.
Application Dilution
WB 1:300-5000
ICC 1:100-500