New to Bioss? Enjoy 35% of your first order. Use code "FirstOrder35" - Offer valid for new U.S. Customers on direct orders only

Annexin A2 Antibody

Applications

  • WB
  • IP
  • ICC

Reactivity

  • Human
Overview
Catalog # bsm-70335M
Product Name Annexin A2 Antibody
Applications WB, IP, ICC
Specificity Clone M009 detects a 36 kDa* protein corresponding to the molecular mass of Annexin A2 on SDS-PAGE immunoblots of human A431, MDA-MB-231, MeWo, HUVEC, and PC3 cells. The antibody also works for ELISA, immunocytochemical labeling, and immunoprecipitation applications. In addition, mass spectrometry analysis of immunoprecipitates using AM0091 in human A431 cell lysates confirms that this antibody only detects Annexin A2 protein.
Reactivity Human
Specifications
Conjugation Unconjugated
Host Mouse
Source Clone M009 was generated from a proprietary antigen related to human Annexin A2 in MDA-MB-231 breast cancer cell line.
Clonality Monoclonal
Clone # M009
Isotype IgG1
Purification Purified by Protein G.
Storage Buffer PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
Storage Condition Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Target
Swiss Prot P07355
Synonyms Lipocortin, Calpactin 1, Annexin II
Background The Annexin family is composed of at least thirteen mammalian genes (Annexin A1-13). These proteins are characterized by a conserved core domain which binds to phospholipids in a Ca2+-dependent manner and a unique amino terminal region which may confer binding specificity. Annexins have roles in membrane fusion, endocytosis, secretion, and repair. Annexin A1 binds to cellular membranes in a calcium-dependent manner, promotes membrane fusion and endocytosis, and has been implicated as an anti-inflammatory mediator. Annexin A2 is a cytoskeletal calcium-dependent phospholipid binding protein, which has been shown to be a mediator of corticosteroid activity, a substrate for serine/threonine kinases and growth regulated tyrosine kinases, and may play a role in secretion. Annexin A5 is a PKC inhibitor, directly interacts with VEGFR2 receptor, and binds phosphatidylserine to inhibit blood coagulation. Annexin A6 reverses transformation of A431 cells after overexpression, and this effect may involve annexin A6 targeting of p120 RasGAP to the plasma membrane to inactivate Ras.
Application Dilution
WB 1:300-5000
IP 1-2ug
ICC 1:100-500