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ALDH1A1 (N-terminal region) Antibody

Applications

  • WB

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bsm-70332M
Product Name ALDH1A1 (N-terminal region) Antibody
Applications WB
Specificity This antibody antibody detects a 55 kDa* protein on SDS-PAGE immunoblots of human A431, HepG2, and mouse liver, as well as human recombinant ALDH1A1. The antibody does not detect ALDH1A3 in PC3 cells, ALDH1A2 in Jurkat cells, and bovine tubulin (55 kDa).
Reactivity Human, Mouse, Rat
Specifications
Conjugation Unconjugated
Host Mouse
Source Clone M558 was generated from a recombinant protein corresponding to amino acids in the N-terminal region from human ALDH1A1. This sequence is highly conserved in rat and mouse ALDH1A1.
Clonality Monoclonal
Clone # M558
Isotype IgG1
Purification Purified by Protein G.
Storage Buffer PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
Storage Condition Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Target
Swiss Prot P00352
Synonyms RALDH, ALDH-E1, ALHDII, Aldehyde dehydrogenase, ALDC, PUMB1, ALDH1A1
Background Aldehyde dehydrogenase (ALDH) superfamily is a ubiquitous group of enzymes found in all taxonomic domains. ALDH detoxifies endogenous and exogenous aldehydes, protecting cellular homeostasis and organismal functions. These enzymes are necessary for the synthesis of retinoic acid, betaine, and folate. Recent studies have reported high levels of ALDH found in cancer cells, suggesting that ALDH can act as a marker for cancer cells found in a wide variety of tissues including skin, prostate, lung, and neural tissues. Additionally, certain diseases can be identified when ALDH activity is absent. ALDH1A1 is vital for retinol synthesis and alcohol metabolism. ALDH1A1 active sites include an active cysteine residue, which catalyses the transformation of aldehydes into their respective carboxylic groups. ALDH1A1 amino acid sequence and function is highly conserved in humans and rodents.
Application Dilution
WB 1:300-5000