COPE Monoclonal Antibody

$\r\nBlocking buffer: 5% NFDM\/TBST\r\nPrimary Ab dilution: 1:20000\r\nPrimary Ab incubation condition: room temperature 2h\r\nSecondary Ab: Goat Anti-Mouse IgG H&L (HRP)\r\nLysate: 1: HeLa, 2: 293, 3: MCF-7\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 10 s\r\nPredicted MW: 32 kDa\r\nObserved MW: 32 kDa$

\r\nBlocking buffer: 5% NFDM\/TBST\r\nPrimary Ab dilution: 1:20000\r\nPrimary Ab incubation condition: room temperature 2h\r\nSecondary Ab: Goat Anti-Mouse IgG H&L (HRP)\r\nLysate: 1: HeLa, 2: 293, 3: MCF-7\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 10 s\r\nPredicted MW: 32 kDa\r\nObserved MW: 32 kDa

Applications

Reactivity

Human

Overview
Catalog #	bsm-60452M
Product Name	COPE Monoclonal Antibody
Applications	WB, ICC
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Mouse
Clonality	Monoclonal
Clone #	H12B9
Isotype	IgG1/Kappa
Concentration	1mg/ml
Purification	Protein G.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Swiss Prot	O14579
Application Dilution
WB	1:300-5000
ICC	1:100-500