ECM1 Monoclonal Antibody

$Paraformaldehyde-fixed, paraffin embedded Human esophagus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with ECM1 Monoclonal Antibody, Unconjugated (bsm-54715R) at 1:50 for 30 minutes at room temperature, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Human esophagus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with ECM1 Monoclonal Antibody, Unconjugated (bsm-54715R) at 1:50 for 30 minutes at room temperature, DAB staining.

$MCF-7 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (ECM1) monoclonal Antibody, Unconjugated (bsm-54715R) 1:50, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.$

MCF-7 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (ECM1) monoclonal Antibody, Unconjugated (bsm-54715R) 1:50, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

$SiHa cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (ECM1) monoclonal Antibody, Unconjugated (bsm-54715R) 1:50, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.$

SiHa cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (ECM1) monoclonal Antibody, Unconjugated (bsm-54715R) 1:50, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Applications

WB
IHC-P
IF(ICC)

Reactivity

Human
Rat

Overview
Catalog #	bsm-54715R
Product Name	ECM1 Monoclonal Antibody
Applications	WB, IHC-P, IF(ICC)
Reactivity	Human, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Synthetic Peptide within N terminal human ECM1.
Clonality	Monoclonal
Clone #	1H7
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	1893
Swiss Prot	Q16610
Subcellular location	Extracellular matrix
Synonyms	ECM 1 antibody, Ecm1 antibody, ECM1_HUMAN antibody, Extracellular matrix protein 1 antibody, Secretory component p85 antibody, URBWD antibody
Background	Extracellular matrix protein 1 (ECM1) This family consists of several eukaryotic extracellular matrix protein 1 (ECM1) sequences. ECM1 has been shown to regulate endochondral bone formation, stimulate the proliferation of endothelial cells and induce angiogenesis. Mutations in the ECM1 gene can cause lipoid proteinosis, a disorder which causes generalised thickening of skin, mucosae and certain viscera. Classical features include beaded eyelid papules and laryngeal infiltration leading to hoarseness.
Application Dilution
WB	1:300-5000
IHC-P	1:200-400
IF(ICC)	1:50-200