SPAK Monoclonal Antibody

$Paraformaldehyde-fixed, paraffin embedded Human prostate cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with SPAK Monoclonal Antibody, Unconjugated (bsm-54711R) at 1:50 for 30 minutes at room temperature, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Human prostate cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with SPAK Monoclonal Antibody, Unconjugated (bsm-54711R) at 1:50 for 30 minutes at room temperature, DAB staining.

$Paraformaldehyde-fixed, paraffin embedded Human pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with SPAK Monoclonal Antibody, Unconjugated (bsm-54711R) at 1:50 for 30 minutes at room temperature, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Human pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with SPAK Monoclonal Antibody, Unconjugated (bsm-54711R) at 1:50 for 30 minutes at room temperature, DAB staining.

Daudi cells were fixed with 4% PFA for 10min at room temperature,permeabilized with for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with SPAK Monoclonal Antibody(bsm-54711R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green).

Applications

WB
FCM
IHC-P

Reactivity

Human

Overview
Catalog #	bsm-54711R
Product Name	SPAK Monoclonal Antibody
Applications	WB, FCM, IHC-P
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Synthetic Peptide corresponding to C terminal human SPAK.
Clonality	Monoclonal
Clone #	6H14
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	27347
Swiss Prot	Q9UEW8
Subcellular location	Cytoplasm, Nucleus
Synonyms	DCHT antibody, DKFZp686K05124 antibody, OTTHUMP00000165175 antibody, PASK antibody, Proline alanine rich STE20 related kinase antibody, Serine threonine kinase 39 (STE20/SPS1 homolog yeast) antibody, Serine threonine kinase 39 antibody, Serine/threonine protein kinase 39 antibody, Serine/threonine-protein kinase 39 antibody, Small intestine SPAK like kinase antibody, SPAK antibody, Ste 20 related kinase antibody, Ste-20-related kinase antibody, Ste20 like protein kinase antibody, STE20/SPS1 homolog antibody, STE20/SPS1 related proline alanine rich protein kinase antibody, STE20/SPS1-related proline-alanine-rich protein kinase antibody, STK 39 antibody, Stk39 antibody, STK39_HUMAN antibody
Background	The phosphorylation and dephosphorylation of proteins on serine and threonine residues is an essential means of regulating a broad range of cellular functions in eukaryotes, including cell division, homeostasis and apoptosis. A group of proteins that are intimately involved in this process are the serine/threonine (Ser/Thr) protein kinases. SPAK, also known as STK39 (serine threonine kinase 39), DCHT or PASK, is a 547 amino acid protein that localizes to both the cytoplasm and the nucleus and contains one protein kinase domain. Expressed predominately in pancreas, brain, heart, lung, liver and testis, SPAK functions as a Ser/Thr protein kinase that catalyzes the ATP-dependent phosphorylation of target proteins and is thought to be involved in mediating stress-activated signals. The gene encoding SPAK maps to human chromosome 2, which houses over 1,400 genes and comprises nearly 8% of the human genome.
Application Dilution
WB	1:300-5000
FCM	1:20-100
IHC-P	1:200-400