PSMA2 Monoclonal Antibody

$Paraformaldehyde-fixed, paraffin embedded Rat cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with PSMA2 Monoclonal Antibody, Unconjugated (bsm-54695R) at 1:200 for 30 minutes at room temperature, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Rat cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with PSMA2 Monoclonal Antibody, Unconjugated (bsm-54695R) at 1:200 for 30 minutes at room temperature, DAB staining.

$Paraformaldehyde-fixed, paraffin embedded Human lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with PSMA2 Monoclonal Antibody, Unconjugated (bsm-54695R) at 1:50 for 30 minutes at room temperature, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Human lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with PSMA2 Monoclonal Antibody, Unconjugated (bsm-54695R) at 1:50 for 30 minutes at room temperature, DAB staining.

$Paraformaldehyde-fixed, paraffin embedded Human liver cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with PSMA2 Monoclonal Antibody, Unconjugated (bsm-54695R) at 1:200 for 30 minutes at room temperature, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Human liver cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with PSMA2 Monoclonal Antibody, Unconjugated (bsm-54695R) at 1:200 for 30 minutes at room temperature, DAB staining.

$Paraformaldehyde-fixed, paraffin embedded Human breast cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with PSMA2 Monoclonal Antibody, Unconjugated (bsm-54695R) at 1:200 for 30 minutes at room temperature, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Human breast cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with PSMA2 Monoclonal Antibody, Unconjugated (bsm-54695R) at 1:200 for 30 minutes at room temperature, DAB staining.

$Paraformaldehyde-fixed, paraffin embedded Human intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with PSMA2 Monoclonal Antibody, Unconjugated (bsm-54695R) at 1:50 for 30 minutes at room temperature, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Human intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with PSMA2 Monoclonal Antibody, Unconjugated (bsm-54695R) at 1:50 for 30 minutes at room temperature, DAB staining.

$Flow cytometric analysis of PSMA2 was done on F9 cells. The cells were fixed, permeabilized and stained with the primary antibody (bsm-54695R,1\/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1\/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).$

Flow cytometric analysis of PSMA2 was done on F9 cells. The cells were fixed, permeabilized and stained with the primary antibody (bsm-54695R,1\/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1\/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Applications

WB
FCM
IHC-P

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-54695R
Product Name	PSMA2 Monoclonal Antibody
Applications	WB, FCM, IHC-P
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Synthetic Peptide within N-terminal human PSMA2.
Clonality	Monoclonal
Clone #	5H8
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	5683
Swiss Prot	P25787
Subcellular location	Cytoplasm, Nucleus
Synonyms	HC3 antibody, Macropain subunit C3 antibody, MU antibody, Multicatalytic endopeptidase complex subunit C3 antibody, PMSA2 antibody, Proteasome (prosome macropain) subunit alpha type 2 antibody, Proteasome alpha 2 subunit antibody, Proteasome component C3 antibody, Proteasome subunit alpha type 2 antibody, Proteasome subunit alpha type-2 antibody, Proteasome subunit HC3 antibody, PSA2_HUMAN antibody, PSC2 antibody, PSC3 antibody, PSMA 2 antibody, psmA2 antibody
Background	The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. PSMA2 may have a potential regulatory effect on another component(s) of the proteasome complex through tyrosine phosphorylation.
Application Dilution
WB	1:300-5000
FCM	1:20-100
IHC-P	1:200-400