CD239 Monoclonal Antibody

$Paraformaldehyde-fixed, paraffin embedded Human kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with CD239 Monoclonal Antibody, Unconjugated (bsm-54684R) at 1:200 for 30 minutes at room temperature, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Human kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with CD239 Monoclonal Antibody, Unconjugated (bsm-54684R) at 1:200 for 30 minutes at room temperature, DAB staining.

$Paraformaldehyde-fixed, paraffin embedded Human placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with CD239 Monoclonal Antibody, Unconjugated (bsm-54684R) at 1:200 for 30 minutes at room temperature, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Human placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with CD239 Monoclonal Antibody, Unconjugated (bsm-54684R) at 1:200 for 30 minutes at room temperature, DAB staining.

$Flow cytometric analysis of CD239 was done on JAR cells. The cells were fixed, permeabilized and stained with the primary antibody (bsm-54684R, 1\/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1\/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).$

Flow cytometric analysis of CD239 was done on JAR cells. The cells were fixed, permeabilized and stained with the primary antibody (bsm-54684R, 1\/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1\/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Applications

WB
FCM
IHC-P

Reactivity

Human

Overview
Catalog #	bsm-54684R
Product Name	CD239 Monoclonal Antibody
Applications	WB, FCM, IHC-P
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Recombinant protein corresponding to C-terminal human CD239
Clonality	Monoclonal
Clone #	8F16
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	4059
Swiss Prot	P50895
Subcellular location	Membrane
Synonyms	Antigen identified by monoclonal antibody F8 antibody, AU antibody, Auberger B antigen antibody, B CAM cell surface glycoprotein antibody, B cell adhesion molecule antibody, B-CAM cell surface glycoprotein antibody, Basal cell adhesion molecule (Lu and Au blood groups) antibody, Basal cell adhesion molecule (Lutheran blood group) antibody, Basal cell adhesion molecule antibody, Basal cell adhesion molecule Lu and Au blood groups antibody, Basal cell adhesion molecule Lutheran blood group antibody, Bcam antibody, BCAM_HUMAN antibody, CD239 antibody, CD239 antigen antibody, F8/G253 antigen antibody, Glycoprotein 95kDa antibody, LU antibody, Lutheran antibody, Lutheran antigen antibody, Lutheran blood group (Auberger b antigen included) antibody, Lutheran blood group Auberger b antigen included antibody, Lutheran blood group glycoprotein antibody, MSK19 antibody
Background	This gene encodes Lutheran blood group glycoprotein, a member of the immunoglobulin superfamily and a receptor for the extracellular matrix protein, laminin. The protein contains five extracellular immunoglobulin domains, a single transmembrane domain, and a short C-terminal cytoplasmic tail. This protein may play a role in epithelial cell cancer and in vaso-occlusion of red blood cells in sickle cell disease. Polymorphisms in this gene define some of the antigens in the Lutheran system and also the Auberger system. Inactivating variants of this gene result in the recessive Lutheran null phenotype, Lu(a-b-), of the Lutheran blood group. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2012].
Application Dilution
WB	1:300-5000
FCM	1:20-100
IHC-P	1:200-400

Applications

Reactivity

Search our catalog of hundreds of thousands of products