ICAD Monoclonal Antibody

$Paraformaldehyde-fixed, paraffin embedded Human thyroid; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with ICAD Monoclonal Antibody, Unconjugated (bsm-54674R) at 1:400 for 30 minutes at room temperature, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Human thyroid; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with ICAD Monoclonal Antibody, Unconjugated (bsm-54674R) at 1:400 for 30 minutes at room temperature, DAB staining.

$Paraformaldehyde-fixed, paraffin embedded Human breast; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with ICAD Monoclonal Antibody, Unconjugated (bsm-54674R) at 1:400 for 30 minutes at room temperature, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Human breast; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with ICAD Monoclonal Antibody, Unconjugated (bsm-54674R) at 1:400 for 30 minutes at room temperature, DAB staining.

$Paraformaldehyde-fixed, paraffin embedded Human intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with ICAD Monoclonal Antibody, Unconjugated (bsm-54674R) at 1:400 for 30 minutes at room temperature, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Human intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with ICAD Monoclonal Antibody, Unconjugated (bsm-54674R) at 1:400 for 30 minutes at room temperature, DAB staining.

$Paraformaldehyde-fixed, paraffin embedded Human pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with ICAD Monoclonal Antibody, Unconjugated (bsm-54674R) at 1:400 for 30 minutes at room temperature, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Human pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with ICAD Monoclonal Antibody, Unconjugated (bsm-54674R) at 1:400 for 30 minutes at room temperature, DAB staining.

$Flow cytometric analysis of ICAD was done on HCT116 cells. The cells were fixed, permeabilized and stained with the primary antibody (bsm-54674R, 1\/100) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-rabbit IgG Secondary antibody at 1\/500 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).$

Flow cytometric analysis of ICAD was done on HCT116 cells. The cells were fixed, permeabilized and stained with the primary antibody (bsm-54674R, 1\/100) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-rabbit IgG Secondary antibody at 1\/500 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Applications

WB
FCM
IHC-P
IHC

Reactivity

Human

Overview
Catalog #	bsm-54674R
Product Name	ICAD Monoclonal Antibody
Applications	WB, FCM, IHC-P, IHC
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Synthetic Peptide within human ICAD aa 1-200.
Clonality	Monoclonal
Clone #	12H9
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Store at -20°C for 12 months.
Target
Swiss Prot	O00273
Subcellular location	Cytoplasm
Synonyms	A330085O09Rik antibody, Caspase activated deoxyribonuclease inhibitor short form antibody, DFF 1 antibody, DFF 45 antibody, DFF alpha antibody, DFF-45 antibody, DFF1 antibody, DFF35 antibody, DFF45 antibody, DFFA antibody, Dffa DNA fragmentation factor, alpha subunit antibody, DFFA_HUMAN antibody, DNA fragmentation factor 45 kDa subunit antibody, DNA Fragmentation Factor Alpha Subunit antibody, DNA fragmentation factor subunit alpha antibody, DNA fragmentation factor, 45 kD, alpha subunit antibody, DNA fragmentation factor, 45kDa, alpha polypeptide (DFFA), transcript variant 1 antibody, DNA fragmentation factor, 45kDa, alpha polypeptide antibody, DNA fragmentation factor, alpha subunit antibody, DNAation factor 45 kDa subunit antibody, H13 antibody, ICAD antibody, ICAD L antibody, ICAD S antibody, Inhibitor of CAD antibody, Inhibitor of Caspase Activated DNase antibody, MGC143066 antibody, OTTHUMP00000001903 antibody, OTTHUMP00000001904 antibody, RP23 121D17.3 antibody
Application Dilution
WB	1:300-5000
FCM	1:20-100
IHC-P	1:200-400
IHC

Applications

Reactivity

Search our catalog of hundreds of thousands of products