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Histone H3 (mono methyl R2) Monoclonal Antibody

Applications

  • WB
  • IF(ICC)

Reactivity

  • Human
  • Mouse
Overview
Catalog # bsm-54537R
Product Name Histone H3 (mono methyl R2) Monoclonal Antibody
Applications WB, IF(ICC)
Reactivity Human, Mouse
Specifications
Conjugation Unconjugated
Host Rabbit
Source Recombinant protein
Modification Site Arg2
Clonality Monoclonal
Clone # 2C3
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition Store at -20°C for 12 months.
Target
Gene ID 8290
Swiss Prot P68431
Subcellular location Nucleus, Chromosome
Synonyms HIST1 cluster, H3J antibody, Histone gene cluster 1, H3 histone family, member E antibody, Histone gene cluster 1, H3G antibody, H3 histone family, member J antibody, HIST1 cluster, H3E antibody, HIST1 cluster, H3I antibody, Histone gene cluster 1, H3C antibody, FLJ92264 antibody, H3 histone family, member A antibody
Background Modulation of the chromatin structure plays an important role in the regulation of transcription in eukaryotes. The nucleosome, made up of four core histone proteins (H2A, H2B, H3 and H4), is the primary building block of chromatin. The N-terminal tail of core histones undergoes different posttranslational modifications including acetylation, phosphorylation and methylation. These modifications occur in response to cell signal stimuli and have a direct effect on gene expression. In most species, the histone H2B is primarily acetylated at lysines 5, 12, 15 and 20. Histone H3 is primarily acetylated at lysines 9, 14, 18 and 23. Acetylation at lysine 9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms. Phosphorylation at Ser10 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis.
Application Dilution
WB 1:300-5000
IF(ICC) 1:50-200