CHD1L (8A1) Monoclonal Antibody

$Lane 1: HepG2 Cells; Probed with CHD1L (8A1) Monoclonal Antibody (bsm-54350R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: HepG2 Cells; Probed with CHD1L (8A1) Monoclonal Antibody (bsm-54350R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

$Paraformaldehyde-fixed and paraffin-embedded Human Tonsil tissue incubated with CHD1L (8A1) Monoclonal Antibody (bsm-54350R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin. The tissue was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins.$

Paraformaldehyde-fixed and paraffin-embedded Human Tonsil tissue incubated with CHD1L (8A1) Monoclonal Antibody (bsm-54350R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin. The tissue was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins.

$Paraformaldehyde-fixed and paraffin-embedded Human Colon Cancer tissue incubated with CHD1L (8A1) Monoclonal Antibody (bsm-54350R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin. The tissue was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins.$

Paraformaldehyde-fixed and paraffin-embedded Human Colon Cancer tissue incubated with CHD1L (8A1) Monoclonal Antibody (bsm-54350R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin. The tissue was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins.

$Paraformaldehyde-fixed and paraffin-embedded Mouse Testis tissue incubated with CHD1L (8A1) Monoclonal Antibody (bsm-54350R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin. The tissue was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins.$

Paraformaldehyde-fixed and paraffin-embedded Mouse Testis tissue incubated with CHD1L (8A1) Monoclonal Antibody (bsm-54350R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin. The tissue was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins.

Flow cytometric analysis of HepG2 cells with CHD1L (8A1) Monoclonal Antibody (bsm-54350R) at a 1:100 dilution (purple) compared with an unlabeled control (cells without incubation with primary antibody; yellow).

Applications

WB
FCM
IHC-P

Reactivity

Human
Mouse

Overview
Catalog #	bsm-54350R
Product Name	CHD1L (8A1) Monoclonal Antibody
Applications	WB, FCM, IHC-P
Reactivity	Human, Mouse
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Recombinant human CHD1L between 600 to 800 amino acids.
Clonality	Monoclonal
Clone #	8A1
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Store at 4C for up to 2 weeks. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Target
Gene ID	9557
Swiss Prot	Q86WJ1
Subcellular location	Nucleus
Synonyms	Chromodomain-helicase-DNA-binding protein 1-like; Amplified in liver cancer protein 1; CHD1L; ALC1
Background	DNA helicase which plays a role in chromatin-remodeling following DNA damage. Targeted to sites of DNA damage through interaction with poly(ADP-ribose) and functions to regulate chromatin during DNA repair. Able to catalyze nucleosome sliding in an ATP-dependent manner. Helicase activity is strongly stimulated upon poly(ADP-ribose)-binding.
Application Dilution
WB	1:300-5000
FCM	1:20-100
IHC-P	1:200-400