PARP2 (19A1) Monoclonal Antibody

Flow cytometric analysis of SH-SY5Y cells with PARP2 (19A1) Monoclonal Antibody (bsm-54344R) at a 1:50 dilution (purple) compared with an unlabeled control (cells without incubation with primary antibody; yellow).

$Lane 1: SiHa Cells; Lane 2: Raji Cells; Probed with PARP2 (19A1) Monoclonal Antibody (bsm-54344R) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: SiHa Cells; Lane 2: Raji Cells; Probed with PARP2 (19A1) Monoclonal Antibody (bsm-54344R) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

Applications

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-54344R
Product Name	PARP2 (19A1) Monoclonal Antibody
Applications	WB, FCM
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Recombinant protein within the C-terminus of human PARP2.
Clonality	Monoclonal
Clone #	19A1
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Store at 4C for up to 2 weeks. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Target
Gene ID	10038
Swiss Prot	Q9UGN5
Subcellular location	Nucleus
Synonyms	Poly [ADP-ribose] polymerase 2; ADP-ribosyltransferase diphtheria toxin-like 2; NAD(+) ADP-ribosyltransferase 2; Poly[ADP-ribose] synthase 2; PARP-2; hPARP-2; ARTD2; ADPRT-2; pADPRT-2; PARP2; ADPRT2; ADPRTL2
Background	Poly-ADP-ribosyltransferase that mediates poly-ADP-ribosylation of proteins and plays a key role in DNA repair. Mainly mediates glutamate and aspartate ADP-ribosylation of target proteins: the ADP-D-ribosyl group of NAD+ is transferred to the acceptor carboxyl group of glutamate and aspartate residues and further ADP-ribosyl groups are transferred to the 2'-position of the terminal adenosine moiety, building up a polymer with an average chain length of 20-30 units. ADP-ribosylation follows DNA damage and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. Also mediates serine ADP-ribosylation of target proteins following interaction with HPF1; HPF1 conferring serine specificity. In addition to proteins, also able to ADP-ribosylate DNA: preferentially acts on 5'-terminal phosphates at DNA strand breaks termini in nicked duplex
Application Dilution
WB	1:300-5000
FCM	1:20-100