MCM5 (9G7) Monoclonal Antibody

$Lane 1: Mouse Thymus; Lane 2: SiHa Cells; Probed with MCM5 (9G7) Monoclonal Antibody (bsm-54262R) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: Mouse Thymus; Lane 2: SiHa Cells; Probed with MCM5 (9G7) Monoclonal Antibody (bsm-54262R) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

$Paraformaldehyde-fixed and paraffin-embedded Human Tonsil tissue incubated with MCM5 (9G7) Monoclonal Antibody (bsm-54262R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human Tonsil tissue incubated with MCM5 (9G7) Monoclonal Antibody (bsm-54262R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Human Placenta tissue incubated with MCM5 (9G7) Monoclonal Antibody (bsm-54262R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human Placenta tissue incubated with MCM5 (9G7) Monoclonal Antibody (bsm-54262R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Mouse Testis tissue incubated with MCM5 (9G7) Monoclonal Antibody (bsm-54262R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Mouse Testis tissue incubated with MCM5 (9G7) Monoclonal Antibody (bsm-54262R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Applications

WB
IHC-P

Reactivity

Human
Mouse

Overview
Catalog #	bsm-54262R
Product Name	MCM5 (9G7) Monoclonal Antibody
Applications	WB, IHC-P
Reactivity	Human, Mouse
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Recombinant protein within C terminal human MCM5.
Clonality	Monoclonal
Clone #	9G7
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Store at 4C for up to 2 weeks. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Target
Gene ID	4174
Swiss Prot	P33992
Subcellular location	Cytoplasm, Nucleus
Synonyms	DNA replication licensing factor MCM5; P1-CDC46; MCM5; CDC46; minichromosome maintenance complex component 5.
Background	Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Interacts with MCMBP.
Application Dilution
WB	1:300-5000
IHC-P	1:200-400