DDX6 (15C2) Monoclonal Antibody

$Lane 1: Human Colon; Lane 2: SH-SY5Y Cells; Lane 3: SiHa Cells; Lane 4: K562 Cells; Probed with DDX6 (15C2) Monoclonal Antibody (bsm-54253R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: Human Colon; Lane 2: SH-SY5Y Cells; Lane 3: SiHa Cells; Lane 4: K562 Cells; Probed with DDX6 (15C2) Monoclonal Antibody (bsm-54253R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

$Paraformaldehyde-fixed and paraffin-embedded Human Tonsil tissue incubated with DDX6 (15C2) Monoclonal Antibody (bsm-54253R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human Tonsil tissue incubated with DDX6 (15C2) Monoclonal Antibody (bsm-54253R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Human Kidney tissue incubated with DDX6 (15C2) Monoclonal Antibody (bsm-54253R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human Kidney tissue incubated with DDX6 (15C2) Monoclonal Antibody (bsm-54253R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Mouse Small Intestine tissue incubated with DDX6 (15C2) Monoclonal Antibody (bsm-54253R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Mouse Small Intestine tissue incubated with DDX6 (15C2) Monoclonal Antibody (bsm-54253R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Rat Brain tissue incubated with DDX6 (15C2) Monoclonal Antibody (bsm-54253R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Rat Brain tissue incubated with DDX6 (15C2) Monoclonal Antibody (bsm-54253R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Applications

WB
IHC-P

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-54253R
Product Name	DDX6 (15C2) Monoclonal Antibody
Applications	WB, IHC-P
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Recombinant protein within human DDX6 aa 100-300
Clonality	Monoclonal
Clone #	15C2
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Store at 4C for up to 2 weeks. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Target
Gene ID	1656
Swiss Prot	P26196
Subcellular location	Cytoplasm, Nucleus
Synonyms	Probable ATP-dependent RNA helicase DDX6; ATP-dependent RNA helicase p54; DEAD box protein 6; Oncogene RCK; DDX6; HLR2; RCK.
Background	RCK, also known as DDX6 and P54, is a member of the DEAD-box RNA helicase family of proteins, all of which share common protein motifs. Found in most tissues, RCK is an unwindase that exhibits ATP-dependent RNA un-winding activity, as well as the ability to decay RNA in the 5'-3' direction. In non-malignant cells, RCK is associated with all processes of normal RNA metabolism including splicing, export and translation initiation. Mutations in the gene encoding RCK can cause the protein to be overexpressed, changing its function to that of an oncogene that positively regulates the expression of genes involved in cell growth and proliferation. It is believed that, through its unwindase activity, the main function of RCK is to downregulate mRNA expression and maintain normal transcriptional levels within the cell.
Application Dilution
WB	1:300-5000
IHC-P	1:200-400