Overview |
bsm-54131R |
DNA Ligase IV (2D9) Monoclonal Antibody |
WB, IHC-P |
Human |
Specifications |
Unconjugated |
Rabbit |
Recombinant protein within human DNA Ligase IV aa 500-700 |
Monoclonal |
2D9 |
IgG |
1ug/ul |
Purified by Protein A. |
Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide. |
Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
Target |
3981 |
P49917 |
Nucleus |
DNA ligase 4; DNA ligase IV; Polydeoxyribonucleotide synthase [ATP] 4; LIG4. |
Efficiently joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The LIG4-XRCC4 complex is responsible for the NHEJ ligation step, and XRCC4 enhances the joining activity of LIG4. Binding of the LIG4-XRCC4 complex to DNA ends is dependent on the assembly of the DNA-dependent protein kinase complex DNA-PK to these DNA ends. |
Application Dilution |
WB |
1:300-5000 |
IHC-P |
1:200-400 |