KPNA2 (2D10) Monoclonal Antibody

$Lane 1: Hela Cells; Lane 2: 293T Cells; Lane 3: HepG2 Cells; Lane 4: NIH-3T3 Cells; Lane 5: PC-12 Cells; Probed with \r\nKPNA2 (2D10) Monoclonal Antibody (bsm-54115R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: Hela Cells; Lane 2: 293T Cells; Lane 3: HepG2 Cells; Lane 4: NIH-3T3 Cells; Lane 5: PC-12 Cells; Probed with \r\nKPNA2 (2D10) Monoclonal Antibody (bsm-54115R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

Flow cytometric analysis of 293T cells with KPNA2 (2D10) Monoclonal Antibody (bsm-54115R) at 1:100 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).

Applications

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-54115R
Product Name	KPNA2 (2D10) Monoclonal Antibody
Applications	WB, FCM
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Recombinant protein within human KPNA2 aa 1-200
Clonality	Monoclonal
Clone #	2D10
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Store at 4C for up to 2 weeks. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Target
Gene ID	3838
Swiss Prot	P52292
Synonyms	Importin subunit alpha-1; Karyopherin subunit alpha-2; RAG cohort protein 1; SRP1-alpha; KPNA2; RCH1; SRP1; QIP2; IPOA1; SRP1alpha; SRP1-alpha.
Background	Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus.
Application Dilution
WB	1:300-5000
FCM	1:20-100