Clathrin heavy chain (2H4) Monoclonal Antibody

$Lane 1: Hela Cells; Lane 2: PC-12 Cells; Probed with Clathrin heavy chain (2H4) Monoclonal Antibody (bsm-54022R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: Hela Cells; Lane 2: PC-12 Cells; Probed with Clathrin heavy chain (2H4) Monoclonal Antibody (bsm-54022R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

$Paraformaldehyde-fixed and paraffin-embedded Human Adrenal Gland tissue incubated with Clathrin heavy chain (2H4) Monoclonal Antibody (bsm-54022R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human Adrenal Gland tissue incubated with Clathrin heavy chain (2H4) Monoclonal Antibody (bsm-54022R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Human Colon Cancer tissue incubated with Clathrin heavy chain (2H4) Monoclonal Antibody (bsm-54022R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human Colon Cancer tissue incubated with Clathrin heavy chain (2H4) Monoclonal Antibody (bsm-54022R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Human Kidney tissue incubated with Clathrin heavy chain (2H4) Monoclonal Antibody (bsm-54022R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human Kidney tissue incubated with Clathrin heavy chain (2H4) Monoclonal Antibody (bsm-54022R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Applications

WB
IHC-P

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-54022R
Product Name	Clathrin heavy chain (2H4) Monoclonal Antibody
Applications	WB, IHC-P
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Human Clathrin heavy chain aa 100-300
Clonality	Monoclonal
Clone #	2H4
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	1213; 8218
Swiss Prot	Q00610; P53675
Synonyms	Clathrin heavy chain 1; Clathrin heavy chain 2; Clathrin heavy chain on chromosome 17; Clathrin heavy chain on chromosome 22; CLH-17; CLH-22; CLTC; CLTCL1; CLTCL2; CLH17; CLH22; CLTCL; CLTD; Clathrin heavy chain like 1; KIAA0034
Background	Clathrin is a major cytosolic coat protein in pits and vesicles originating from the plasma membrane and the trans-Golgi network. In receptor-mediated endocytosis, receptor proteins are engulfed by clathrin-coated vesicles. Clathrin is composed of three heavy chains and three light chains which associate non-covalently to form a triskelion structure. Clathrin heavy chain (HC) is composed of a terminal globular domain, a distal segment and a proximal segment containing a light chain binding site. The proximal segment of the Clathrin HC protein is essential for interactions between clathrin heavy chains and light chains which result in the formation of the triskelion structure.
Application Dilution
WB	1:300-5000
IHC-P	1:200-400