Glucose 6 Phosphate Dehydrogenase (4F7) Monoclonal Antibody

Flow cytometric analysis of Hela cells with Glucose 6 Phosphate Dehydrogenase (4F7) Monoclonal Antibody (bsm-54011R) at a 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).

$IF(ICC) staining with Glucose 6 Phosphate Dehydrogenase (4F7) Monoclonal Antibody (bsm-54011R) at 1:100 in MCF-7 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with Glucose 6 Phosphate Dehydrogenase (4F7) Monoclonal Antibody (bsm-54011R) at 1:100 in MCF-7 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

$Paraformaldehyde-fixed and paraffin-embedded Human liver tissue incubated with Glucose 6 Phosphate Dehydrogenase (4F7) Monoclonal Antibody (bsm-54011R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human liver tissue incubated with Glucose 6 Phosphate Dehydrogenase (4F7) Monoclonal Antibody (bsm-54011R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Human stomach cancer tissue incubated with Glucose 6 Phosphate Dehydrogenase (4F7) Monoclonal Antibody (bsm-54011R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human stomach cancer tissue incubated with Glucose 6 Phosphate Dehydrogenase (4F7) Monoclonal Antibody (bsm-54011R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Mouse Skeletal Muscle tissue incubated with Glucose 6 Phosphate Dehydrogenase (4F7) Monoclonal Antibody (bsm-54011R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Mouse Skeletal Muscle tissue incubated with Glucose 6 Phosphate Dehydrogenase (4F7) Monoclonal Antibody (bsm-54011R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Mouse Testis tissue incubated with Glucose 6 Phosphate Dehydrogenase (4F7) Monoclonal Antibody (bsm-54011R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Mouse Testis tissue incubated with Glucose 6 Phosphate Dehydrogenase (4F7) Monoclonal Antibody (bsm-54011R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Applications

WB
FCM
IHC-P
IHC-F
IF(IHC-P)
IF(ICC)

Reactivity

Human
Mouse

Overview
Catalog #	bsm-54011R
Product Name	Glucose 6 Phosphate Dehydrogenase (4F7) Monoclonal Antibody
Applications	WB, FCM, IHC-P, IHC-F, IF(IHC-P), IF(ICC)
Reactivity	Human, Mouse
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Human Glucose 6 Phosphate Dehydrogenase aa 1-300
Clonality	Monoclonal
Clone #	4F7
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	2539
Swiss Prot	P11413
Subcellular location	Cytoplasm, Nucleus, Cell membrane, Cytoplasmic domain
Synonyms	Glucose-6-phosphate 1-dehydrogenase; G6PD; G6PD1
Background	Catalyzes the rate-limiting step of the oxidative pentose-phosphate pathway, which represents a route for the dissimilation of carbohydrates besides glycolysis. The main function of this enzyme is to provide reducing power (NADPH) and pentose phosphates for fatty acid and nucleic acid synthesis.
Application Dilution
WB	1:300-5000
FCM	1:20-100
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(ICC)	1:50-200