Ku70 (6D1) Monoclonal Antibody

$Lane 1: Hela cells lysates; Lane 2: Jurkat cells lysates; Probed with Ku 70 (6D1) Monoclonal Antibody (bsm-52970R) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: Hela cells lysates; Lane 2: Jurkat cells lysates; Probed with Ku 70 (6D1) Monoclonal Antibody (bsm-52970R) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

Flow cytometric analysis of Hela cells with Ku70 (6D1) Monoclonal Antibody (bsm-52970R) at a 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).

$Paraformaldehyde-fixed and paraffin-embedded Human breast cancer tissue incubated with Ku 70 (6D1) Monoclonal Antibody (bsm-52970R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human breast cancer tissue incubated with Ku 70 (6D1) Monoclonal Antibody (bsm-52970R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Human tonsil tissue incubated with Ku 70 (6D1) Monoclonal Antibody (bsm-52970R) at 1:30, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human tonsil tissue incubated with Ku 70 (6D1) Monoclonal Antibody (bsm-52970R) at 1:30, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$IF(ICC) staining with Ku 70 (6D1) Monoclonal Antibody (bsm-52970R) at 1:100 in A549 cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with Ku 70 (6D1) Monoclonal Antibody (bsm-52970R) at 1:100 in A549 cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

$IF(ICC) staining with Ku 70 (6D1) Monoclonal Antibody (bsm-52970R) at 1:100 in Hela cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with Ku 70 (6D1) Monoclonal Antibody (bsm-52970R) at 1:100 in Hela cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

Applications

WB
FCM
IHC-P
IF(ICC)

Reactivity

Human

Overview
Catalog #	bsm-52970R
Product Name	Ku70 (6D1) Monoclonal Antibody
Applications	WB, FCM, IHC-P, IF(ICC)
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Human ku70 between 500-600 amino acids
Clonality	Monoclonal
Clone #	6D1
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	2547
Swiss Prot	P12956
Synonyms	X-ray repair cross-complementing protein 6, 5'-deoxyribose-5-phosphate lyase Ku70, 70 kDa subunit of Ku antigen, ATP-dependent DNA helicase 2 subunit 1, ATP-dependent DNA helicase II 70 kDa subunit, CTC box-binding factor 75 kDa subunit, DNA repair protein XRCC6, Lupus Ku autoantigen protein p70, Thyroid-lupus autoantigen, X-ray repair complementing defective repair in Chinese hamster cells 6, 5'-dRP lyase Ku70, CTC75, CTCBF, Ku70, TLAA, XRCC6
Background	Single-stranded DNA-dependent ATP-dependent helicase. Has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3'-5' direction. Binding to DNA may be mediated by XRCC6. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The XRCC5/6 dimer acts as regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA by 100-fold. The XRCC5/6 dimer is probably involved in stabilizing broken DNA ends and bringing them together. The assembly of the DNA-PK complex to DNA ends is required for the NHEJ ligation step. Required for osteocalcin gene expression. Probably also acts as a 5'-deoxyribose-5-phosphate lyase (5'-dRP lyase), by catalyzing the beta-elimination of the 5' deoxyribose-5-phosphate at an abasic site near double-strand breaks. 5'-dRP lyase activity allows to 'clean' the termini of abasic sites, a class of nucleotide damage commonly associated with strand breaks, before such broken ends can be joined. The XRCC5/6 dimer together with APEX1 acts as a negative regulator of transcription. Plays a role in the regulation of DNA virus-mediated innate immune response by assembling into the HDP-RNP complex, a complex that serves as a platform for IRF3 phosphorylation and subsequent innate immune response activation through the cGAS-STING pathway.
Application Dilution
WB	1:300-5000
FCM	1:20-100
IHC-P	1:200-400
IF(ICC)	1:50-200