MyoD1 (4C4) Monoclonal Antibody

$Hela cells lysates probed with MyoD1 (4C4) Monoclonal Antibody (bsm-52966R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.\r\n\r\n$

Hela cells lysates probed with MyoD1 (4C4) Monoclonal Antibody (bsm-52966R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.\r\n\r\n

Flow cytometric analysis of Hela cells with MyoD1 (4C4) Monoclonal Antibody (bsm-52966R) at a 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).

Applications

WB
FCM
IHC-P

Reactivity

Human

Overview
Catalog #	bsm-52966R
Product Name	MyoD1 (4C4) Monoclonal Antibody
Applications	WB, FCM, IHC-P
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Human MyoD1 between 200-600 amino acids
Clonality	Monoclonal
Clone #	4C4
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	4654
Swiss Prot	P15172
Synonyms	Myoblast determination protein 1, Class C basic helix-loop-helix protein 1, Myogenic factor 3, bHLHc1, Myf-3, MYOD1
Background	Acts as a transcriptional activator that promotes transcription of muscle-specific target genes and plays a role in muscle differentiation. Together with MYF5 and MYOG, co-occupies muscle-specific gene promoter core region during myogenesis. Induces fibroblasts to differentiate into myoblasts. Interacts with and is inhibited by the twist protein. This interaction probably involves the basic domains of both proteins (By similarity).
Application Dilution
WB	1:300-5000
FCM	1:20-100
IHC-P	1:200-400