Ras (2C4) Monoclonal Antibody

$Lane 1: 293T lysates; Lane 2: MCF-7 lysates; Lane 3: Hela lysates; Lane 4: Zebra fish lysates; Probed with Ras (2C4) Monoclonal Antibody (bsm-52871R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.\r\n\r\n$

Lane 1: 293T lysates; Lane 2: MCF-7 lysates; Lane 3: Hela lysates; Lane 4: Zebra fish lysates; Probed with Ras (2C4) Monoclonal Antibody (bsm-52871R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.\r\n\r\n

$IF(ICC) staining with Ras (2C4) Monoclonal Antibody (bsm-52871R) at 1:200 in Hela cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with Ras (2C4) Monoclonal Antibody (bsm-52871R) at 1:200 in Hela cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

$IF(ICC) staining with Ras (2C4) Monoclonal Antibody (bsm-52871R) at 1:200 in MCF-7 cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with Ras (2C4) Monoclonal Antibody (bsm-52871R) at 1:200 in MCF-7 cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

Applications

WB
IHC-P
IF(ICC)

Reactivity

Human
Mouse
Rat
Zebrafish

Overview
Catalog #	bsm-52871R
Product Name	Ras (2C4) Monoclonal Antibody
Applications	WB, IHC-P, IF(ICC)
Reactivity	Human, Mouse, Rat, Zebrafish
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Human Ras aa 1-100
Clonality	Monoclonal
Clone #	2C4
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	4893
Swiss Prot	P01111
Synonyms	GTPase NRas, Transforming protein N-Ras, GTPase HRas, H-Ras-1, Ha-Ras, Transforming protein p21, c-H-ras, p21ras, GTPase KRas, K-Ras 2, Ki-Ras, c-K-ras, c-Ki-ras, NRAS, HRAS, KRAS
Background	N-RAS (neuroblastoma RAS viral oncogene homolog) analogously to other GTP-binding proteins (such as Translation Elongation Factor EFTu or signal transducing G-Proteins) RAS proteins are involved in signal transduction pathways, ras gene family is part of the ras superfamily including the mammalian RAS, RAL, RAC, RHO, RAP, and RAB gene families and the yeast homologs like SEC4 and YPT1 genes; genes encode small monomeric proteins of low molecular mass (20-30 kDa) which share at least 30% homology to RAS proteins.
Application Dilution
WB	1:300-5000
IHC-P	1:200-400
IF(ICC)	1:50-200