pro Caspase 9 (3T6) Monoclonal Antibody

Flow cytometric analysis of Jurkat cells with pro Caspase 9 (3T6) Monoclonal Antibody (bsm-52719R) at 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).

$Hela cells Probed with pro Caspase 9 (3T6) Monoclonal Antibody (bsm-52719R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Hela cells Probed with pro Caspase 9 (3T6) Monoclonal Antibody (bsm-52719R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

Applications

Reactivity

Human

Overview
Catalog #	bsm-52719R
Product Name	pro Caspase 9 (3T6) Monoclonal Antibody
Applications	WB, FCM
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Human Caspase-9 between 250 to 350 amino acids
Clonality	Monoclonal
Clone #	3T6
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	842
Swiss Prot	P55211
Synonyms	Caspase-9, Apoptotic protease Mch-6, Apoptotic protease-activating factor 3, ICE-like apoptotic protease 6, CASP-9, APAF-3, ICE-LAP6, CASP9
Background	Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Promotes DNA damage-induced apoptosis in a ABL1/c-Abl-dependent manner. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP). Isoform 2 lacks activity is an dominant-negative inhibitor of caspase-9.
Application Dilution
WB	1:300-5000
FCM	1:20-100