AMPK beta 1 (6C1) Monoclonal Antibody

Flow cytometric analysis of A431 cells with AMPK beta 1 (6C1) Monoclonal Antibody (bsm-52680R) at 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).

$A431 cell lysate probed with AMPK beta 1 (6C1) Monoclonal Antibody (bsm-52680R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

A431 cell lysate probed with AMPK beta 1 (6C1) Monoclonal Antibody (bsm-52680R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

Applications

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-52680R
Product Name	AMPK beta 1 (6C1) Monoclonal Antibody
Applications	WB, FCM
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Human AMPK beta 1 between 150-250 amino acids
Clonality	Monoclonal
Clone #	6C1
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	5564
Swiss Prot	Q9Y478
Synonyms	5'-AMP-activated protein kinase subunit beta-1, AMPK subunit beta-1, AMPKb, PRKAB1
Background	Non-catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Beta non-catalytic subunit acts as a scaffold on which the AMPK complex assembles, via its C-terminus that bridges alpha (PRKAA1 or PRKAA2) and gamma subunits (PRKAG1, PRKAG2 or PRKAG3).
Application Dilution
WB	1:300-5000
FCM	1:20-100