E2F2 (4A6) Monoclonal Antibody

Flow cytometric analysis of K562 cells with E2F2 (4A6) Monoclonal Antibody (bsm-52641R) at a 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).

$Lane 1: K562 Cells; Lane 2: 293T Cells; Lane 3: HepG2 Cells; Probed with E2F2 (4A6) Monoclonal Antibody (bsm-52641R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: K562 Cells; Lane 2: 293T Cells; Lane 3: HepG2 Cells; Probed with E2F2 (4A6) Monoclonal Antibody (bsm-52641R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

Applications

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-52641R
Product Name	E2F2 (4A6) Monoclonal Antibody
Applications	WB, FCM
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Recombinant Human E2F2 1 to 110 amino acids
Clonality	Monoclonal
Clone #	4A6
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	1870
Swiss Prot	Q14209
Synonyms	Transcription factor E2F2, E2F-2, E2F2
Background	Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from g1 to s phase. E2F2 binds specifically to RB1 in a cell-cycle dependent manner.
Application Dilution
WB	1:300-5000
FCM	1:20-100