USP5 Monoclonal Antibody
Applications
Predicted Reactivity
Overview | |
Catalog # | bsm-51588M |
Product Name | USP5 Monoclonal Antibody |
Applications | WB |
Predicted Reactivity | Human, Mouse, Rat |
Specifications | |
Conjugation | Unconjugated |
Host | Mouse |
Source | Recombinant human USP5. |
Clonality | Monoclonal |
Clone # | H4Y13 |
Isotype | IgG1 _ |
Concentration | 0.5ug/ul |
Purification | Purified by Protein G. |
Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
Storage Condition | Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
Target | |
Gene ID | 8078 |
Swiss Prot | P45974 |
Subcellular location | Cytoplasm, Nucleus |
Synonyms | Deubiquitinating enzyme 5; Isopeptidase T; ISOT 1; ISOT; Ubiquitin carboxyl-terminal hydrolase 5; Ubiquitin isopeptidase T; Ubiquitin specific peptidase 5 (isopeptidase T); Ubiquitin specific peptidase 5; Ubiquitin specific processing protease 5; Ubiquitin specific protease 5 (isopeptidase T); Ubiquitin specific protease 5 (ubiquitin isopeptidase T); Ubiquitin thioesterase 5; Ubiquitin thiolesterase 5; Ubiquitin-specific-processing protease 5; UBP5_HUMAN; USP-5; USP 5 |
Background | Cleaves linear and branched multiubiquitin polymers with a marked preference for branched polymers. Involved in unanchored 'Lys-48'-linked polyubiquitin disassembly. Binds linear and 'Lys-63'-linked polyubiquitin with a lower affinity. Knock-down of USP5 causes the accumulation of p53/TP53 and an increase in p53/TP53 transcriptional activity because the unanchored polyubiquitin that accumulates is able to compete with ubiquitinated p53/TP53 but not with MDM2 for proteasomal recognition. |
Application Dilution | |
WB | =1:500-2000, |