LIMK1 Monoclonal Antibody

$Lane 1: Human Brain tissue lysates; Lane 2: Mouse Brain tissue lysates probed with LIMK1 Monoclonal Antibody, Unconjugated (bsm-51477M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Human Brain tissue lysates; Lane 2: Mouse Brain tissue lysates probed with LIMK1 Monoclonal Antibody, Unconjugated (bsm-51477M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

Predicted Reactivity

Human
Mouse

Overview
Catalog #	bsm-51477M
Product Name	LIMK1 Monoclonal Antibody
Applications	WB
Predicted Reactivity	Human, Mouse
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	KLH conjugated synthetic peptide derived from human LIMK1
Immunogen Range	459-508/647
Clonality	Monoclonal
Clone #	L6K5
Concentration	0.5ug/ul
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	3984
Swiss Prot	P53667
Subcellular location	Cytoplasm, Nucleus
Synonyms	EC 2.7.11.1; LIM domain kinase 1; LIM motif-containing protein kinase; LIMK; LIMK-1; LIMK2; LIMK-2; LIM domain kinase 2; LIMK1_HUMAN.
Background	Serine/threonine-protein kinase that plays an essential role in the regulation of actin filament dynamics. Acts downstream of several Rho family GTPase signal transduction pathways. Activated by upstream kinases including ROCK1, PAK1 and PAK4, which phosphorylate LIMK1 on a threonine residue located in its activation loop. LIMK1 subsequently phosphorylates and inactivates the actin binding/depolymerizing factors cofilin-1/CFL1, cofilin-2/CFL2 and destrin/DSTN, thereby preventing the cleavage of filamentous actin (F-actin), and stabilizing the actin cytoskeleton. In this way LIMK1 regulates several actin-dependent biological processes including cell motility, cell cycle progression, and differentiation. Phosphorylates TPPP on serine residues, thereby promoting microtubule disassembly. Stimulates axonal outgrowth and may be involved in brain development. Isoform 3 has a dominant negative effect on actin cytoskeletal changes.
Application Dilution
WB	=1:500-1000Ôºå