Overview |
bsm-51459M |
NANOG Monoclonal Antibody |
WB, IHC-P, IHC-F |
Human |
Specifications |
Unconjugated |
Mouse |
Recombinant human NANOG. |
Monoclonal |
N3F1 |
0.5ug/ul |
0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
Target |
79923 |
Q80Z64 |
Nucleus |
NANOG_MOUSE; Embryonic stem cell specific homeobox protein (Nanog); ENK; FLJ12581; FLJ40451; Homeobox transcription factor Nanog; Nanog homeobox; Homeobox protein NANOG; ES cell-associated protein 4; Early embryo specific expression NK-type homeobox protein; Homeobox transcription factor Nanog; Nanog; Ecat4. |
Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes. Acts as a transcriptional activator or repressor. Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3'. When overexpressed, promotes cells to enter into S phase and proliferation. |
Application Dilution |
WB |
=1:500-2000,IHC-P=1:400-800,IHC-F=1:400-800, |
IHC-P |
1:200-400 |
IHC-F |
1:100-500 |