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IRF3 (2C4) Monoclonal Antibody

Applications

  • WB
  • FCM
  • IHC-P
  • IF(ICC)
  • IF

Reactivity

  • Human
Overview
Catalog # bsm-51312M
Product Name IRF3 (2C4) Monoclonal Antibody
Applications WB, FCM, IHC-P, IF(ICC), IF
Reactivity Human
Specifications
Conjugation Unconjugated
Host Mouse
Source This IRF3 antibody is generated from a mouse immunized with a recombinant protein.
Clonality Monoclonal
Clone # 2C4
Isotype IgG1
Concentration 0.5ug/ul
Purification Purified by Protein G.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 3661
Swiss Prot Q14653
Synonyms IIAE7; Interferon regulatory factor 3; IRF-3; IRF3
Background Key transcriptional regulator of type I interferon (IFN)-dependent immune responses which plays a critical role in the innate immune response against DNA and RNA viruses. Regulates the transcription of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimulated genes (ISG) by binding to an interferon-stimulated response element (ISRE) in their promoters. Acts as a more potent activator of the IFN-beta (IFNB) gene than the IFN-alpha (IFNA) gene and plays a critical role in both the early and late phases of the IFNA/B gene induction. Found in an inactive form in the cytoplasm of uninfected cells and following viral infection, double-stranded RNA (dsRNA), or toll-like receptor (TLR) signaling, is phosphorylated by IKBKE and TBK1 kinases. This induces a conformational change, leading to its dimerization and nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of the type I IFN and ISG genes. Can activate distinct gene expression programs in macrophages and can induce significant apoptosis in primary macrophages.
Application Dilution
WB 1:300-5000
FCM 1:20-100
IHC-P 1:200-400
IF(ICC) 1:50-200
IF