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ATG4A (14C9) Monoclonal Antibody

Applications

  • WB
  • IHC-P
  • IF(ICC)
  • IF

Reactivity

  • Human
Overview
Catalog # bsm-51292M
Product Name ATG4A (14C9) Monoclonal Antibody
Applications WB, IHC-P, IF(ICC), IF
Reactivity Human
Specifications
Conjugation Unconjugated
Host Mouse
Source This ATG4A antibody is generated from a mouse immunized with a recombinant protein.
Clonality Monoclonal
Clone # 14C9
Isotype IgG2b
Concentration 0.5ug/ul
Purification Purified by Protein G.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C for 12 months.
Target
Gene ID 115201
Swiss Prot Q8WYN0
Synonyms APG4A; AUTL2; Cysteine protease ATG4A; AUT-like 2 cysteine endopeptidase; Autophagin-2; Autophagy-related cysteine endopeptidase 2; Autophagy-related protein 4 homolog A; hAPG4A; ATG4A
Background Cysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy. Cleaves the C-terminal amino acid of ATG8 family proteins to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. Preferred substrate is GABARAPL2 followed by MAP1LC3A and GABARAP. Has also an activity of delipidating enzyme for the PE-conjugated forms.
Application Dilution
WB 1:300-5000
IHC-P 1:200-400
IF(ICC) 1:50-200
IF