ANGPTL8 Monoclonal Antibody

$Paraformaldehyde-fixed, paraffin embedded Mouse liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with ANGPTL8 Monoclonal Antibody, Unconjugated (bsm-49019M) at 1:200 overnight at 4\u00b0C, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Mouse liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with ANGPTL8 Monoclonal Antibody, Unconjugated (bsm-49019M) at 1:200 overnight at 4\u00b0C, DAB staining.

$Lane 1: Human HepG2 cell lysates; Lane 2: Human MCF-7 cell lysates probed with ANGPTL8 Monoclonal Antibody, Unconjugated (bsm-49019M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Human HepG2 cell lysates; Lane 2: Human MCF-7 cell lysates probed with ANGPTL8 Monoclonal Antibody, Unconjugated (bsm-49019M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(ICC)

Reactivity

Mouse

Overview
Catalog #	bsm-49019M
Product Name	ANGPTL8 Monoclonal Antibody
Applications	ELISA, IHC-P, IHC-F, IF(IHC-P), IF(ICC)
Reactivity	Mouse
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	Recombinant mouse ANGPTL8
Immunogen Range	16-198/198
Clonality	Monoclonal
Clone #	22G2
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein G.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	624219
Swiss Prot	Q8R1L8
Subcellular location	Secreted
Synonyms	C19orf80; ANGL8_HUMAN; Angiopoietin-like protein 8; Betatrophin; Lipasin; Refeeding-induced fat and liver protein; PRO1185; PVPA599; RIFL; TD26; Chromosome 19 open reading frame 80; hepatocellular carcinoma-associated gene TD26; Hepatocellular carcinoma-associated protein TD26.
Background	Hormone that acts as a blood lipid regulator by regulating serum triglyceride levels (PubMed:22569073, PubMed:22809513, PubMed:23150577). May be involved in the metabolic transition between fasting and refeeding: required to direct fatty acids to adipose tissue for storage in the fed state.
Application Dilution
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(ICC)	1:50-200