C1orf19 Polyclonal Antibody

Lane 1: 293T cell lysates probed with C1orf19 Polyclonal Antibody, Unconjugated (bs-9783R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

$Paraformaldehyde-fixed, paraffin embedded Rat kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with C1orf19 Polyclonal Antibody, Unconjugated (bs-9783R) at 1:200 overnight at 4\u00b0C, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Rat kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with C1orf19 Polyclonal Antibody, Unconjugated (bs-9783R) at 1:200 overnight at 4\u00b0C, DAB staining.

$Paraformaldehyde-fixed, paraffin embedded Rat uterus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with C1orf19 Polyclonal Antibody, Unconjugated (bs-9783R) at 1:200 overnight at 4\u00b0C, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Rat uterus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with C1orf19 Polyclonal Antibody, Unconjugated (bs-9783R) at 1:200 overnight at 4\u00b0C, DAB staining.

$Paraformaldehyde-fixed, paraffin embedded Mouse kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with C1orf19 Polyclonal Antibody, Unconjugated (bs-9783R) at 1:200 overnight at 4\u00b0C, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Mouse kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with C1orf19 Polyclonal Antibody, Unconjugated (bs-9783R) at 1:200 overnight at 4\u00b0C, DAB staining.

Applications

WB
IHC-P
IF(IHC-P)

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bs-9783R
Product Name	C1orf19 Polyclonal Antibody
Applications	WB, IHC-P, IF(IHC-P)
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human C1orf19/SEN15
Immunogen Range	51-150/171
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Synonyms	Chromosome 1 open reading frame 19; Chromosome 1 open reading frame 19, isoform CRA_c; HsSen15; sen15; SEN15 homolog; SEN15_HUMAN; tRNA intron endonuclease Sen15; tRNA splicing endonuclease 15 homolog S. cerevisiae; tRNA splicing endonuclease subunit Sen15; tRNA-intron endonuclease SEN15; tRNA-splicing endonuclease subunit SEN15; Tsen15.
Background	SEN15, also known as TSEN15 (tRNA-splicing endonuclease subunit Sen15) or C1orf19, is a nuclear protein that plays a role in tRNA splicing. Widely expressed with highest expression in testis and uterus, SEN15 is a non-catalytic subunit of the multi-protein tRNA-splicing endonuclease complex. The endonuclease complex is responsible for identifying and cleaving pre-tRNA at both 5? and 3? splice sites, thereby releasing introns and free tRNA molecules with 2',3' cyclic phosphates and 5'-OH termini. In addition to its role in pre-tRNA splicing, the endonuclease complex participates in mRNA processing and, via its association with pre-mRNA processing factors, is thought to link pre-tRNA and pre-mRNA splicing events. As a subunit of the complex, SEN15 participates in protein expression and, ultimately, cell growth and division.
Application Dilution
WB	1:300-5000
IHC-P	1:200-400
IF(IHC-P)	1:50-200