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Caspase-8 subunit p18 Antibody, PE Conjugated

Applications

  • WB
  • FCM

Reactivity

  • Human
  • Mouse
  • Rat

Predicted Reactivity

  • Dog
  • Cow
  • Pig
  • Horse
Overview
Catalog # bs-6463R-PE
Product Name Caspase-8 subunit p18 Antibody, PE Conjugated
Applications WB, FCM
Reactivity Human, Mouse, Rat
Predicted Reactivity Dog, Cow, Pig, Horse
Specifications
Conjugation PE
Host Rabbit
Source KLH conjugated synthetic peptide derived from human Caspase-8 subunit p18
Immunogen Range 188-280/479
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 841
Swiss Prot Q14790
Subcellular location Cytoplasm
Synonyms CAP4; MACH; MCH5; FLICE; ALPS2B; Casp-8; Caspase-8; Apoptotic cysteine protease; Apoptotic protease Mch-5; FADD-homologous ICE/ced-3-like protease; FADD-like ICE; ICE-like apoptotic protease 5; MORT1-associated ced-3 homolog; CASP8
Background Most upstream protease of the activation cascade of caspases responsible for the TNFRSF6/FAS mediated and TNFRSF1A induced cell death. Binding to the adapter molecule FADD recruits it to either receptor. The resulting aggregate called death-inducing signaling complex (DISC) performs CASP8 proteolytic activation. The active dimeric enzyme is then liberated from the DISC and free to activate downstream apoptotic proteases. Proteolytic fragments of the N-terminal propeptide (termed CAP3, CAP5 and CAP6) are likely retained in the DISC. Cleaves and activates CASP3, CASP4, CASP6, CASP7, CASP9 and CASP10. May participate in the GZMB apoptotic pathways. Cleaves ADPRT. Hydrolyzes the small-molecule substrate, Ac-Asp-Glu-Val-Asp-|-AMC. Likely target for the cowpox virus CRMA death inhibitory protein. Isoform 5, isoform 6, isoform 7 and isoform 8 lack the catalytic site and may interfere with the pro-apoptotic activity of the complex.
Application Dilution
WB 1:300-5000
FCM 1:20-100