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ERK5 (Ser731+Thr733) Polyclonal Antibody, AbBy Fluor® 594 Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human

Predicted Reactivity

  • Mouse
  • Rat
  • Dog
  • Cow
  • Rabbit
Overview
Catalog # bs-5486R-BF594
Product Name ERK5 (Ser731+Thr733) Polyclonal Antibody, AbBy Fluor® 594 Conjugated
Applications WB, IF(IHC-P), IF(IHC-F), IF(ICC)
Specificity Recognizes Ser721+Thr723 in mice and rats
Reactivity Human
Predicted Reactivity Mouse, Rat, Dog, Cow, Rabbit
Specifications
Conjugation AbBy Fluor® 594
Host Rabbit
Source KLH conjugated synthetic phosphopeptide derived from human ERK5 around the phosphorylation sites of Ser731+Thr733
Modification Site Ser731+Thr733
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 5598
Swiss Prot Q13164
Subcellular location Cytoplasm, Nucleus
Synonyms BMK1; ERK4; ERK5; PRKM7; Mitogen-activated protein kinase 7; MAP kinase 7; MAPK 7; Big MAP kinase 1; BMK-1; Extracellular signal-regulated kinase 5; ERK-5; MAPK7
Background Plays a role in various cellular processes such as proliferation, differentiation and cell survival. The upstream activator of MAPK7 is the MAPK kinase MAP2K5. Upon activation, it translocates to the nucleus and phosphorylates various downstream targets including MEF2C. EGF activates MAPK7 through a Ras-independent and MAP2K5-dependent pathway. May have a role in muscle cell differentiation. May be important for endothelial function and maintenance of blood vessel integrity. MAP2K5 and MAPK7 interact specifically with one another and not with MEK1/ERK1 or MEK2/ERK2 pathways. Phosphorylates SGK1 at Ser-78 and this is required for growth factor-induced cell cycle progression. Involved in the regulation of p53/TP53 by disrupting the PML-MDM2 interaction.
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200