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MEK5(Ser129) Polyclonal Antibody, PE-Cy5 Conjugated

Applications

  • WB

Reactivity

  • Human

Predicted Reactivity

  • Cow
  • Sheep
  • Horse
  • Rabbit
Overview
Catalog # bs-5431R-PE-Cy5
Product Name MEK5(Ser129) Polyclonal Antibody, PE-Cy5 Conjugated
Applications WB
Reactivity Human
Predicted Reactivity Cow, Sheep, Horse, Rabbit
Specifications
Conjugation PE-Cy5
Host Rabbit
Source KLH conjugated synthetic phosphopeptide derived from human MEK5 around the phosphorylation site of Ser129
Modification Site Ser129
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 5607
Subcellular location Cytoplasm
Synonyms MAP2K5phospho S129; Dual specicity mitogen activated protein kinase kinase 5; MAP kinase kinase 5; MAP kinase kinase MEK5b; MAP2K5; MAPK/ERK kinase 5; MAPKK5; PRKMK5; EC 2.7.12.2.
Background MEK5 is a dual specificity protein kinase belonging to thr Ser/Thr protein kinase family, (MAP kinase kinase family). It is activated by phosphorylation on Ser/Thr by MAP kinase kinases and interacts specifically with ERK5, and not with another MAP kinase like P38. This kinase specifically interacts with and activates MAPK7/ERK5. This kinase itself can be phosphorylated and activated by MAP3K3/MEKK3, as well as by atypical protein kinase C isoforms (aPKCs). It is not phosphorylated by RAFA, RAFB or RAFC and it may interact with GTPases such as CDC42. The signal cascade mediated by this kinase is involved in growth factor stimulated cell proliferation and muscle cell differentiation. MEK5 is expressed in many adult tissues and is most abundant in heart and skeletal muscle.
Application Dilution
WB 1:300-5000