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DGCR8 Polyclonal Antibody

Applications

  • ELISA

Reactivity

  • Human
  • Mouse
Overview
Catalog # bs-53088R
Product Name DGCR8 Polyclonal Antibody
Applications ELISA
Reactivity Human, Mouse
Specifications
Conjugation Unconjugated
Host Rabbit
Source Polyclonal antibody raised in rabbit against mouse DGCR8 using two KLH-conjugated synthetic peptides containing a sequence from the N-terminal and the central region of the protein, respectively
Clonality Polyclonal
Isotype Not Determined
Concentration Not Determined
Purification Whole antiserum from rabbit containing 0.05% azide
Storage Buffer Serum with 0.05% sodium azide.
Storage Condition Store at -20°C for 12 months.
Target
Gene ID 54487
Swiss Prot Q8WYQ5
Synonyms DGCRK6, C22orf12, Microprocessor complex subunit DGCR8, DGCR8, LP4941
Background Component of the microprocessor complex that acts as a RNA- and heme-binding protein that is involved in the initial step of microRNA (miRNA) biogenesis. Component of the microprocessor complex that is required to process primary miRNA transcripts (pri-miRNAs) to release precursor miRNA (pre-miRNA) in the nucleus. Within the microprocessor complex, DGCR8 function as a molecular anchor necessary for the recognition of pri-miRNA at dsRNA-ssRNA junction and directs DROSHA to cleave 11 bp away form the junction to release hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic DICER to generate mature miRNAs (PubMed:26027739, PubMed:26748718). The heme-bound DGCR8 dimer binds pri-miRNAs as a cooperative trimer (of dimers) and is active in triggering pri-miRNA cleavage, whereas the heme-free DGCR8 monomer binds pri-miRNAs as a dimer and is much less active. Both double-stranded and single-stranded regions of a pri-miRNA are required for its binding (PubMed:15531877, PubMed:15574589, PubMed:15589161, PubMed:16751099, PubMed:16906129, PubMed:16963499, PubMed:17159994). Specifically recognizes and binds N6-methyladenosine (m6A)-containing pri-miRNAs, a modification required for pri-miRNAs processing (PubMed:25799998). Involved in the silencing of embryonic stem cell self-renewal.
Application Dilution
ELISA 1:500-1000