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Surface Layer Protein Polyclonal Antibody, Biotin Conjugated

Applications

  • ELISA
  • IHC-P
  • IHC-F

Reactivity

  • Bacteria
Overview
Catalog # bs-3797R-Biotin
Product Name Surface Layer Protein Polyclonal Antibody, Biotin Conjugated
Applications ELISA, IHC-P, IHC-F
Reactivity Bacteria
Specifications
Conjugation Biotin
Host Rabbit
Source KLH conjugated synthetic peptide derived from L, acidophilus S-layer protein
Immunogen Range 301-400/444
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C for 12 months.
Target
Gene ID 3252774
Swiss Prot P35829
Subcellular location Secreted
Synonyms S-layer; Surface layer; surface layer protein; Slp; SLAP_LACAC; S-layer protein; slpA; slpH; Lactobacillus surface layer protein;
Background One of the most fascinating cell envelope structures in prokaryotic organisms is two-dimensional arrays of protein or glycoprotein subunits, termed S-layers (Surface Layers). They are composed of numerous identical subunits forming a symmetric, porous, lattice-like layer that completely covers the cell surface. The subunits are held together and attached to cell wall carbohydrates by non-covalent interactions, and they spontaneously reassemble in vitro by an entropy-driven process. In lactobacilli, S-layer proteins have been detected on many but not all species. Lactobacillus S-layer proteins differ from those of other bacteria in their smaller size and high predicted pI. The positive charge in Lactobacillus S-layer proteins is concentrated in the more conserved cell wall binding domain, which can be either N- or C-terminal depending on the species. The more variable domain is responsible for the self-assembly of the monomers to a periodic structure. The biological functions of Lactobacillus S-layer proteins are poorly understood, but in some species S-layer proteins mediate bacterial adherence to host cells or extracellular matrix proteins or have protective or enzymatic functions. Lactobacillus S-layer proteins show potential for use as antigen carriers in live oral vaccine design because of their adhesive and immunomodulatory properties and the general non-pathogenicity of the species.
Application Dilution
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500