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AMPK beta 1 (Ser182) Polyclonal Antibody, PE Conjugated

Applications

  • WB
  • FCM

Reactivity

  • Human
  • Mouse
  • Rat
  • Others

Predicted Reactivity

  • Dog
  • Cow
  • Horse
  • Rabbit
Overview
Catalog # bs-3027R-PE
Product Name AMPK beta 1 (Ser182) Polyclonal Antibody, PE Conjugated
Applications WB, FCM
Reactivity Human, Mouse, Rat, Others
Predicted Reactivity Dog, Cow, Horse, Rabbit
Specifications
Conjugation PE
Host Rabbit
Source KLH conjugated synthetic phosphopeptide derived from human AMPK beta 1 around the phosphorylation site of Ser182
Modification Site Ser182
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 5564
Swiss Prot Q9Y478
Subcellular location Cytoplasm, Nucleus
Synonyms AMPK; HAMPKb; 5'-AMP-activated protein kinase subunit beta-1; AMPK subunit beta-1; AMPKb; PRKAB1
Background Non-catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Beta non-catalytic subunit acts as a scaffold on which the AMPK complex assembles, via its C-terminus that bridges alpha (PRKAA1 or PRKAA2) and gamma subunits (PRKAG1, PRKAG2 or PRKAG3).
Application Dilution
WB 1:300-5000
FCM 1:20-100