AKR1B1 Polyclonal Antibody

Formalin-fixed and paraffin embedded rat tongue labeled with Anti-AKR1B1 Polyclonal Antibody, Unconjugated (bs-2405R) followed by conjugation to the secondary antibody

$Lane 1: Mouse Adrenal gland tissue lysates; Lane 2: Mouse Testis tissue lysates; Lane 3: Rat Adrenal gland tissue lysates; Lane 4: Rat Testis tissue lysates; Lane 5: Human HepG2 cell lysates; Lane 6: Human A549 cell lysates; Lane 7: Human U87MG cell lysates probed with AKR1B1 Polyclonal Antibody, Unconjugated (bs-2405R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Mouse Adrenal gland tissue lysates; Lane 2: Mouse Testis tissue lysates; Lane 3: Rat Adrenal gland tissue lysates; Lane 4: Rat Testis tissue lysates; Lane 5: Human HepG2 cell lysates; Lane 6: Human A549 cell lysates; Lane 7: Human U87MG cell lysates probed with AKR1B1 Polyclonal Antibody, Unconjugated (bs-2405R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)
ICC

Reactivity

Human
Mouse
Rat

Predicted Reactivity

Dog
Cow
Rabbit

Overview
Catalog #	bs-2405R
Product Name	AKR1B1 Polyclonal Antibody
Applications	WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC
Specificity	This antibody may have a secondary cross-reactivity with AKR1C1/2/3/4 due to a 75% sequence similarity in the corresponding immunogen regions.
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Dog, Cow, Rabbit
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human AKR1B1
Immunogen Range	151-250/316
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	231
Swiss Prot	P15121
Subcellular location	Cytoplasm
Synonyms	AR; ADR; ALR2; ALDR1; Aldose reductase; Aldehyde reductase; Aldo-keto reductase family 1 member B1; AKR1B1
Background	Catalyzes the NADPH-dependent reduction of a wide variety of carbonyl-containing compounds to their corresponding alcohols with a broad range of catalytic efficiencies.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200
ICC	1:100-500