SPDYE3 Polyclonal Antibody

Lane 1: Mouse testis lysates;Lane 2: TT cell lysates; Lane 3: DU145 cell lysates probed with SPDYE3 Polyclonal Antibody, Unconjugated (bs-17464R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

$Lane 1: Human HeLa cell lysates probed with SPDYE3 Polyclonal Antibody, Unconjugated (bs-17646R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.\r\n$

Lane 1: Human HeLa cell lysates probed with SPDYE3 Polyclonal Antibody, Unconjugated (bs-17646R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.\r\n

Applications

WB
ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)
ICC

Reactivity

Human
Mouse

Overview
Catalog #	bs-17646R
Product Name	SPDYE3 Polyclonal Antibody
Applications	WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC
Reactivity	Human, Mouse
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human SPDYE3
Immunogen Range	431-530/549
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	441272
Swiss Prot	A6NKU9
Subcellular location	Cytoplasm, Nucleus
Synonyms	MGC119294; MGC57359; Putative Speedy protein E3; Putative WBSCR19 like protein 7; SPDE3_HUMAN; SPDYE3; Speedy homolog E3; Speedy protein E3.
Background	SPDYE3 (Speedy/RINGO Cell Cycle Regulator Family Member E3) is a Protein-Coding gene. Among its related pathways are Oocyte meiosis. An important paralog of this gene is SPDYE5.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200
ICC	1:100-500