ESD Polyclonal Antibody

$Paraformaldehyde-fixed, paraffin embedded Mouse pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with ESD Polyclonal Antibody, Unconjugated (bs-14633R) at 1:200 overnight at 4\u00b0C, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Mouse pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with ESD Polyclonal Antibody, Unconjugated (bs-14633R) at 1:200 overnight at 4\u00b0C, DAB staining.

Applications

WB
ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)
ICC

Reactivity

Mouse

Predicted Reactivity

Human
Rat
Dog
Pig
Horse

Overview
Catalog #	bs-14633R
Product Name	ESD Polyclonal Antibody
Applications	WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC
Reactivity	Mouse
Predicted Reactivity	Human, Rat, Dog, Pig, Horse
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human ESD
Immunogen Range	7-110/282
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	2098
Swiss Prot	P10768
Subcellular location	Cytoplasm
Synonyms	EC 3.1.2.12; Es-10; Es10; ESD; ESTD_HUMAN; Esterase 10; Esterase D; Esterase D formylglutathione hydrolase; FGH; FGH, included; FLJ11763; Methylumbelliferyl acetate deacetylase; MGC139609; OTTHUMP00000018374; OTTHUMP00000040929; S formylglutathione hydrolase; S-formylglutathione hydrolase; S-formylglutathione hydrolase, included; Sid 478; sid478.
Background	Serine hydrolase involved in the detoxification of formaldehyde.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200
ICC	1:100-500