GALNT1/GalNAc-T1 Polyclonal Antibody

Applications

WB
ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)
ICC

Predicted Reactivity

Human
Mouse
Rat
Dog
Cow
Sheep
Pig
Horse
Chicken

Overview
Catalog #	bs-13273R
Product Name	GALNT1/GalNAc-T1 Polyclonal Antibody
Applications	WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC
Predicted Reactivity	Human, Mouse, Rat, Dog, Cow, Sheep, Pig, Horse, Chicken
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human GALNT1/GalNAc-T1
Immunogen Range	101-200/559
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	2589
Subcellular location	Cytoplasm, Secreted, Cell membrane
Synonyms	GALNAC T1; GalNAc transferase 1; GalNAc-T1; GALNT 1; GALNT1; GALT1_HUMAN; Polypeptide GalNAc transferase 1; Polypeptide N acetylgalactosaminyltransferase 1; Polypeptide N-acetylgalactosaminyltransferase 1 soluble form; pp GaNTase 1; pp-GaNTase 1; Protein UDP acetylgalactosaminyltransferase 1; Protein-UDP acetylgalactosaminyltransferase 1; UDP GalNAc:polypeptide N acetylgalactosaminyltransferase 1; UDP N acetyl alpha D galactosamine:polypeptide N acetylgalactosaminyltransferase 1 GalNAc T1; UDP N acetyl alpha D galactosamine:polypeptide N acetylgalactosaminyltransferase 1; UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 1.
Background	The UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase (GalNAc-T) family of enzymes are substrate-specific proteins that catalyze the transfer of GalNAc (N-acetylgalactosamine) to serine and threonine residues onto various proteins, thereby initiating mucin-type O-linked glycosylation in the Golgi apparatus. GalNAc-T1, also known as GALNT1 (Polypeptide N-acetylgalactosaminyltransferase 1), is a ubiquitously expressed 559 amino acid single-pass type II membrane protein that localizes to the Golgi apparatus and, like other GalNAc-Ts, contains a stem region and a C-terminal ricin/lectin-like domain. GalNAc-T1 catalyzes the first reaction in O-linked oligosaccharide biosynthesis, namely the transfer of an N-acetyl-D-galactosamine residue to a protein acceptor. GalNAc-T1 uses calcium and manganese as cofactors. Due to alternative splicing events, two GalNAc-T1 isoforms are expressed.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200
ICC	1:100-500