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EED Polyclonal Antibody, AbBy Fluor® 750 Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Xenopus laevis
  • Xenopus tropicalis
  • Medaka fish

Predicted Reactivity

  • Human
  • Mouse
  • Rat
  • Cow
  • Sheep
  • Pig
  • Horse
  • Chicken
  • Rabbit
  • Zebrafish
Overview
Catalog # bs-13053R-BF750
Product Name EED Polyclonal Antibody, AbBy Fluor® 750 Conjugated
Applications WB, IF(IHC-P), IF(IHC-F), IF(ICC)
Reactivity Xenopus laevis, Xenopus tropicalis, Medaka fish
Predicted Reactivity Human, Mouse, Rat, Cow, Sheep, Pig, Horse, Chicken, Rabbit, Zebrafish
Specifications
Conjugation AbBy Fluor® 750
Host Rabbit
Source KLH conjugated synthetic peptide derived from human EED
Immunogen Range 51-150/441
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 8726
Subcellular location Nucleus
Synonyms eed; EED protein; EED_HUMAN; Embryonic ectoderm development; Embryonic ectoderm development isoform a; Embryonic ECTODERM development protein homolog; hEED; OTTHUMP00000235483; OTTHUMP00000235484; OTTHUMP00000235485; Polycomb protein eed; WAIT 1; WAIT-1; WAIT1; WD protein associating with integrin cytoplasmic tails 1.
Background The transcriptional repressing Polycomb-group (PcG) and transcriptional activating trithorax-group (trxG) genes of Drosophila are part of a cellular memory system responsible for the stable inheritance of gene activity. PcG proteins assemble into multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. EED (embryonic ectoderm development) is the human homolog of Eed, a murine PcG gene homologous to the Drosophila homeotic gene, extra sex combs. The human EED protein is 99. 5% identical to the mouse EED protein and contains seven WD repeats, which are involved in protein-protein interactions. There are two human EED transcripts that contain a putative 407-nucleotide-long intron and give rise to two HEED protein isoforms, 535 and 494 amino acids in length. EED interacts in a highly specific manner, both in vitro and in vivo, with histone deacetylase (HDAC) proteins.
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200