TRIB1/GIG2 Polyclonal Antibody

$Lane 1: Mouse Thyroid gland tissue lysates; Lane 2: Rat Thyroid gland tissue lysates probed with TRIB1 Polyclonal Antibody, Unconjugated (bs-12343R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Mouse Thyroid gland tissue lysates; Lane 2: Rat Thyroid gland tissue lysates probed with TRIB1 Polyclonal Antibody, Unconjugated (bs-12343R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)
ICC

Predicted Reactivity

Human
Mouse
Rat
Cow
Sheep
Pig
Horse
Rabbit
Monkey

Overview
Catalog #	bs-12343R
Product Name	TRIB1/GIG2 Polyclonal Antibody
Applications	WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC
Predicted Reactivity	Human, Mouse, Rat, Cow, Sheep, Pig, Horse, Rabbit, Monkey
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human TRIB1/GIG2
Immunogen Range	151-250/372
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	10221
Subcellular location	Cytoplasm, Nucleus
Synonyms	Tribbles homolog 1;TRB-1;G-protein-coupled receptor-induced gene 2 protein;GIG-2;SKIP1; TRIB1_HUMAN.
Background	TRB-1 is a 372 amino acid protein that contains one protein kinase domain and belongs to the Ser/Thr protein kinase superfamily. Expressed ubiquitously with highest expression in bone marrow, thyroid gland, skeletal muscle and pancreas, TRB-1 interacts with MAPK kinases and is thought to regulate the activation of MAP kinses, possibly controlling MAP kinase cascades. The gene encoding TRB-1 maps to human chromosome 8, which consists of nearly 146 million base pairs, houses more than 800 genes and is associated with a variety of diseases and malignancies.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200
ICC	1:100-500