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AKT1/2/3 (Ser472/Ser473/Ser474) Antibody, HRP Conjugated

Applications

  • WB
  • ELISA
  • IHC-P
  • IHC-F

Reactivity

  • Human
  • Mouse
  • Rat
  • Bovine
  • Chicken
  • Rabbit
  • Fish
  • Others

Predicted Reactivity

  • Dog
  • Cow
  • Sheep
Overview
Catalog # bs-0876R-HRP
Product Name AKT1/2/3 (Ser472/Ser473/Ser474) Antibody, HRP Conjugated
Applications WB, ELISA, IHC-P, IHC-F
Reactivity Human, Mouse, Rat, Bovine, Chicken, Rabbit, Fish, Others
Predicted Reactivity Dog, Cow, Sheep
Specifications
Conjugation HRP
Host Rabbit
Source KLH conjugated synthetic phosphopeptide derived from human AKT1 around the phosphorylation site of Ser473
Modification Site Ser473
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 24185
Swiss Prot P47196
Subcellular location Cytoplasm, Nucleus, Cell membrane
Synonyms Akt; RAC-alpha serine/threonine-protein kinase; Protein kinase B; PKB; Protein kinase B alpha; PKB alpha; RAC-PK-alpha; Akt1
Background AKT1-specific substrates have been recently identified, including palladin (PALLD), which phosphorylation modulates cytoskeletal organization and cell motility; prohibitin (PHB), playing an important role in cell metabolism and proliferation; and CDKN1A, for which phosphorylation at 'Thr-145' induces its release from CDK2 and cytoplasmic relocalization. These recent findings indicate that the AKT1 isoform has a more specific role in cell motility and proliferation. Phosphorylates CLK2 thereby controlling cell survival to ionizing radiation.
Application Dilution
WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500