New to Bioss? Enjoy 35% of your first order. Use code "FirstOrder35" - Offer valid for new U.S. Customers on direct orders only

EGFR Polyclonal Antibody, PE-Cy5 Conjugated

Applications

  • WB
  • FCM

Reactivity

  • Human

Predicted Reactivity

  • Mouse
  • Rat
  • Dog
  • Pig
Overview
Catalog # bs-0165R-PE-Cy5
Product Name EGFR Polyclonal Antibody, PE-Cy5 Conjugated
Applications WB, FCM
Reactivity Human
Predicted Reactivity Mouse, Rat, Dog, Pig
Specifications
Conjugation PE-Cy5
Host Rabbit
Source KLH conjugated synthetic peptide derived from human EGFR
Immunogen Range 951-1050/1210
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 1956
Swiss Prot P00533
Subcellular location Cytoplasm, Nucleus, Secreted, Cell membrane
Synonyms ERBB; HER1; mENA; ERBB1; PIG61; NISBD2; Epidermal growth factor receptor; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1; EGFR
Background Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. May also activate the NF-kappa-B signaling cascade. Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling. Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin. Isoform 2 may act as an antagonist of EGF action.
Application Dilution
WB 1:300-5000
FCM 1:20-100