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Primary Antibodies

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Catalog #1: bsm-2156M-Cy5

Applications: WB, IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: Cy5

Catalog #1: bsm-2156M-FITC

Applications: WB, IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: FITC

Catalog #1: bsm-2156M-Cy7

Applications: WB, IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: Cy7

Catalog #1: bsm-2182M-Biotin

Applications: ELISA, IHC-P

Reactivity: Others

Conjugate: Biotin

Catalog #1: bsm-2182M-HRP

Applications: ELISA, IHC-P

Reactivity: Others

Conjugate: HRP

Catalog #1: bsm-2182M

Applications: ELISA, IHC-P, IF(IHC-P)

Reactivity: Others

Conjugate: Unconjugated

Catalog #1: bsm-2182M-Cy3

Applications: IF(IHC-P)

Reactivity: Others

Conjugate: Cy3

Catalog #1: bsm-2182M-Cy5

Applications: IF(IHC-P)

Reactivity: Others

Conjugate: Cy5

Catalog #1: bsm-2182M-Cy7

Applications: IF(IHC-P)

Reactivity: Others

Conjugate: Cy7

Catalog #1: bsm-2182M-FITC

Applications: IF(IHC-P)

Reactivity: Others

Conjugate: FITC

Catalog #1: bsm-2271M-Biotin

Applications: WB, ELISA

Reactivity: Virus, Others

Conjugate: Biotin

Catalog #1: bsm-2271M-HRP

Applications: WB, ELISA

Reactivity: Virus, Others

Conjugate: HRP

Catalog #1: bsm-2279M

Applications: WB, IHC-P, IF(IHC-P)

Reactivity: Human, Rat, Rabbit

Conjugate: Unconjugated

Catalog #1: bsm-2271M

Applications: WB, ELISA

Reactivity: Virus, Others

Conjugate: Unconjugated

Catalog #1: bsm-2279M-Cy5

Applications: WB, IF(IHC-P)

Reactivity: Human, Rat, Rabbit

Conjugate: Cy5

Catalog #1: bsm-2279M-Biotin

Applications: WB, IHC-P

Reactivity: Human, Rat, Rabbit

Conjugate: Biotin

Catalog #1: bsm-2279M-HRP

Applications: WB, IHC-P

Reactivity: Human, Rat, Rabbit

Conjugate: HRP

Catalog #1: bsm-2279M-Cy3

Applications: WB, IF(IHC-P)

Reactivity: Human, Rat, Rabbit

Conjugate: Cy3

Catalog #1: bsm-2279M-Cy7

Applications: WB, IF(IHC-P)

Reactivity: Human, Rat, Rabbit

Conjugate: Cy7

Catalog #1: bsm-2279M-FITC

Applications: WB, IF(IHC-P)

Reactivity: Human, Rat, Rabbit

Conjugate: FITC

Catalog #1: bsm-33085M-HRP

Applications: WB, IHC-P

Reactivity: Human

Conjugate: HRP

Paraformaldehyde-fixed, paraffin embedded Human brain glioma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with C-erbB-2\/HER2 Monoclonal Antibody, Unconjugated (bsm-33085M) at 1:400 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human breast cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with C-erbB-2\/HER2 Monoclonal Antibody, Unconjugated (bsm-33085M) at 1:400 overnight at 4\u00b0C, DAB staining.
A549 cell lysates probed with C-erbB-2\/HER2 Polyclonal Antibody, Unconjugated (bsm-33085M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Human MCF-7 cell lysates; Lane 2: Human HeLa cell lysates probed with C-erbB-2\/HER2 Polyclonal Antibody, Unconjugated (bsm-33085M) at 1:500 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bsm-33085M

Applications: WB, FCM, IHC-P

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-33085M-Biotin

Applications: WB, IHC-P

Reactivity: Human

Conjugate: Biotin

A549 cell lysates probed with TUBB3 Polyclonal Antibody, Unconjugated (bsm-33177M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
MCF-7 cell lysates probed with TUBB3 Polyclonal Antibody, Unconjugated (bsm-33177M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with TUBB3 Monoclonal Antibody, Unconjugated (bsm-33177M) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with TUBB3 Monoclonal Antibody, Unconjugated (bsm-33177M) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bsm-33177M

Applications: WB, IHC-P

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-33177M-Biotin

Applications: WB, IHC-P

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bsm-33177M-HRP

Applications: WB, IHC-P

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Catalog #1: bsm-33236M-Biotin

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bsm-33236M

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-33236M-HRP

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Paraformaldehyde-fixed, paraffin embedded Human brain glioma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Bax Monoclonal Antibody, Unconjugated (bsm-33279M) at 1:300 overnight at 4\u00b0C, followed by Goat Anti-Mouse IgG (H+L) Cy3 conjugated secondary antibody. DAPI staining of the nucleus was done and then detected.
Lane 1: Cerebrum lysates probed with Bax Monoclonal Antibody, Unconjugated (bsm-33279M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Tissue\/cell: Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (Bax) monoclonal Antibody, Unconjugated (bs-33279M) 1:100, 90 minutes at 37\u00b0C; followed by a CY3 conjugated Goat Anti-Mouse IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (Bax ) Monoclonal Antibody, Unconjugated (bsm-33279M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.

Catalog #1: bsm-33279M

Applications: WB, IHC-P, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat, Pig

Conjugate: Unconjugated

Catalog #1: bsm-33279M-Biotin

Applications: WB, IHC-P

Reactivity: Human, Mouse, Rat, Pig

Conjugate: Biotin

Catalog #1: bsm-33279M-HRP

Applications: WB, IHC-P

Reactivity: Human, Mouse, Rat, Pig

Conjugate: HRP

Catalog #1: bsm-33279M-Cy5

Applications: WB, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat, Pig

Conjugate: Cy5

Catalog #1: bsm-33279M-Cy3

Applications: WB, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat, Pig

Conjugate: Cy3

Catalog #1: bsm-33279M-Cy7

Applications: WB, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat, Pig

Conjugate: Cy7

Catalog #1: bsm-33279M-FITC

Applications: WB, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat, Pig

Conjugate: FITC

Lane 1: A549 cell lysates;Lane 2: MCF-7 cell lysates; Lane 3: U87MG cell lysates probed with Gamma Tubulin Polyclonal Antibody, Unconjugated (bsm-33313M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin-embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Gamma Tubulin Monoclonal Antibody, Unconjugated (bsm-33313M) at 1:2000 overnight at 4\u00b0C, followed by DAB staining.
Paraformaldehyde-fixed, paraffin-embedded (Rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (Gamma Tubulin) Monoclonal Antibody, Unconjugated (bsm-33313M) at 1:2000 overnight at 4\u00b0C, followed by DAB staining.
Hela cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Gamma Tubulin Monoclonal Antibody(bsm-33313M)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bsm-33313M

Applications: WB, IHC-P, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-33313M-Biotin

Applications: WB, IHC-P

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bsm-33313M-HRP

Applications: WB, IHC-P

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Catalog #1: bsm-33314M

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-33314M-Biotin

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bsm-33314M-HRP

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Paraformaldehyde-fixed, paraffin embedded Mouse liver tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with alpha Tubulin (4G1) monoclonal Antibody, Unconjugated (bsm-50179M) at 1:500 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining
RSC96 probed with alpha Tubulin (4G1) Monoclonal Antibody, Unconjugated (bsm-50179M) at 1:100 for 30 minutes followed by incubation with a FITC conjugated secondary (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).
Paraformaldehyde-fixed, paraffin embedded Human cervical carcinoma tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with alpha Tubulin (4G1) monoclonal Antibody, Unconjugated (bsm-50179M) at 1:500 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining
Lane 1: Hela; Lane 2: 293T; Lane 3: MDA-MB-231; Lane 4: A549; Lane 5: A431; Lane 6: RSC96; Lane 7: VSC4.1; Lane 8: ASPC-1; Lane 9: B16; Lane 10: BV2; Lane 11: H9C2; Lane 12: Hep2G;Lane 13: K562; Lane 14: U937; probed with alpha Tubulin (4G1) Monoclonal Antibody, unconjugated (bsm-50179M) at 1:2000 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 60 minutes at 37\u00b0C.\r\n

Catalog #1: bsm-50179M

Applications: WB, FCM, IHC-P

Reactivity: Human, Mouse, Rat, All

Conjugate: Unconjugated

Lane 1 : HeLa Cell Lysate; Lane 2 : A431 Cell Lysate; Lane 3 : C6 Cell Lysate; probed with bsm-50240M p90RSK1 (8C12) Monoclonal Antibody at 2.0ug\/mL (1:500) and incubated at 4\u2103 overnight, followed by secondary antibody incubation for 60min at room temperature.
Immunoprecipitation of p90RSK1 from A431 cell line using p90RSK1 (8C12) Monoclonal Antibody (bsm-50240M). Lane 1 : A431 Cell Lysate; Lane 2 : Precipitated from 200ug of A431 Cell Lysate using 2ug of antibody; Lane 3 : Precipitated from 200ug of A431 Cell Lysate using 5ug of antibody; Lane 4 : Precipitated from PBS using 5ug of antibody. WB analysis was performed using bsm-50240M at 1.0ug\/mL (1:1000) and incubated at 4\u2103 overnight, followed by secondary antibody incubation for 60min at room temperature.

Catalog #1: bsm-50240M

Applications: WB, ELISA

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bsm-50258M

Applications: WB, ELISA, IHC, IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-33050M-HRP

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Catalog #1: bsm-33050M-Biotin

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bsm-33050M

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated